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List of restriction enzyme cutting sites: L–N

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List of restriction enzyme cutting sites: L–N

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Legend of nucleobases
Code	Nucleotide represented
A	Adenine (A)
C	Cytosine (C)
G	Guanine (G)
T	Thymine (T)
N	A, C, G or T
M	A or C
R	A or G
W	A or T
Y	C or T
S	C or G
K	G or T
H	A, C or T
B	C, G or T
V	A, C or G
D	A, G or T

This article contains a list of the most studied restriction enzymes whose names start with L to N inclusive. It contains approximately 120 enzymes.

The following information is given:

Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature, and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action.
Source: Organism that naturally produces the enzyme.
Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site.
Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:

as in this not listed enzyme:

EcoR70I

Whole list navigation

Restriction enzymes

L

Enzyme PDB code Source Recognition sequence Cut Isoschizomers

LcaI Lactobacillus casei 5' ATCGAT
3' TAGCTA 5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5' BdiI, Bli41I, Bsa29I, BscI, Bsp106I, BspDI, Bst28I, LplI, PgaI, ZhoI

LlaAI Lactococcus lactis cremoris W9 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' Bme12I, Bsp67I, BstENII, ChaI, FnuEI, MgoI, NphI, RalF40I

LlaBI Lactococcus lactis cremoris W56 5' CTRYAG
3' GAYRTC 5' ---C TRYAG--- 3'
3' ---GAYRT C--- 5'

LlaCI Lactococcus lactis W15 5' AAGCTT
3' TTCGAA 5' ---A AGCTT--- 3'
3' ---TTCGA A--- 5'

LlaG2I

Lactococcus lactis cremoris

5' GCTAGC
3' CGATCG

5' ---G CTAGC--- 3'
3' ---CGATC G--- 5'

AceII, AsuNHI, BmtI,	BspOI,
NheI, PstNHI

Lmu60I Listeria murrayi 60 5' CCTNAGG
3' GGANTCC 5' ---CC TNAGG--- 3'
3' ---GGANT CC--- 5' AocI, AxyI, BspR7I, CvnI, Lmu60I, MstII, SauI, SshAI

LplI Lactobacillus plantarum 5' ATCGAT
3' TAGCTA 5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5' BanIII, BbvAII, BdiI, Bsa29I, BscI, BstNZ169I, ClaI, LcaI, PgaI

LpnI

Legionella pneumophila

5' RGCGCY
3' YCGCGR

5' ---RGC GCY--- 3'
3' ---YCG CGR--- 5'

AccB2I,	BfoI,	Bme142I,
Bsp143II, BstH2I, HaeII

LspI Lactobacillus sp. 5' TTCGAA
3' AAGCTT 5' ---TT CGAA--- 3'
3' ---AAGC TT--- 5' Asp10HI, BimI, BsiCI, BstBI, Csp45I, PlaII, PpaAI, Ssp1I

LweI Listeria welshimeri RFL131 5' GCATC
3' CGTAG 5' ---GCATCN₄N NNNN--- 3'
3' ---CGTAGN₄NNNNN --- 5'

M

Enzyme PDB code Source Recognition sequence Cut Isoschizomers

MabI Microbacterium arborescens SE 5' ACCWGGT
3' TGGWCCA 5' ---A CCWGGT--- 3'
3' ---TGGWCC A--- 5'

MaeI Methanococcus aeolicus PL-15/H 5' CTAG
3' GATC 5' ---C TAG--- 3'
3' ---GAT C--- 5'

MaeII Methanococcus aeolicus 5' ACGT
3' TGCA 5' ---A CGT--- 3'
3' ---TGC A--- 5'

MaeIII Methanococcus aeolicus 5' GTNAC
3' CANTG 5' --- GTNAC--- 3'
3' ---CANTG --- 5'

MaeK81I Microcystis aeruginosa K-81 5' CGTACG
3' GCATGC 5' ---C GTACG--- 3'
3' ---GCATG C--- 5'

MaeK81II Microcystis aeruginosa 5' GGNCC
3' CCNGG 5' ---G GNCC--- 3'
3' ---CCNG G--- 5' AsuI, Bal228I, Bce22I, BsiZI, BspF4I, Cfr13I, Nsp7121I, Sau96I

MamI Microbacterium ammoniaphilum 5' GATN₄ATC
3' CTAN₄TAG 5' ---GATNN NNATC--- 3'
3' ---CTANN NNTAG--- 5'

MavI Mycobacterium avium 5' CTCGAG
3' GAGCTC 5' ---C TCGAG--- 3'
3' ---GAGCT C--- 5' BspAAI, BstVI, MlaAI, PaeR7I, SauLPII, SciI, SlaI, Sol10179I, XhoI

MbiI Moraxella bovis Fr1-022 5' CCGCTC
3' GGCGAG 5' ---CCG CTC--- 3'
3' ---GGC GAG--- 5' AccBSI, BsrBI, BstD102I, Bst31NI

MboI Moraxella bovis 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' AspMDI, Bsp105I, BspFI, BstMBI, CviAI, Kzo9I, NdeII, Sth368I

MboII Moraxella bovis 5' GAAGA
3' CTTCT 5' ---GAAGAN₆NN --- 3'
3' ---CTTCTN₆N N--- 5'

MchI Micromonospora chalcea 5' GGCGCC
3' CCGCGG 5' ---GG CGCC--- 3'
3' ---CCGC GG--- 5'

MchAI Mycobacterium chelonae 5' GCGGCCGC
3' CGCCGGCG 5' ---GC GGCCGC--- 3'
3' ---CGCCGG CG--- 5'

MchAII Mycobacterium chelonae 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

McrI Micrococcus cryophilus 5' CGRYCG
3' GCYRGC 5' ---CGRY CG--- 3'
3' ---GC YRGC--- 5'

MfeI Mycoplasma fermentans 5' CAATTG
3' GTTAAC 5' ---C AATTG--- 3'
3' ---GTTAA C--- 5'

MflI Microbacterium flavum 5' RGATCY
3' YCTAGR 5' ---R GATCY--- 3'
3' ---YCTAG R--- 5'

MfoAI Mycobacterium fortuitum TMC 1529 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

Mgl14481I Merismopedia glauca 1448-1 5' CCSGG
3' GGSCC 5' ---CC SGG--- 3'
3' ---GGS CC--- 5' AseII, AsuC2I, BcnI, BpuMI, CauII, Eco1831I, EcoHI, HgiS22I, NciI

MgoI Mycobacterium gordonae 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' AspMDI, BspAI, BstENII, ChaI, FnuEI, MkrAI, NphI, Sau3AI

MhaAI Mycobacterium habana 206 5' CTGCAG
3' GACGTC 5' ---CTGCA G--- 3'
3' ---G ACGTC--- 5' AliAJI, Asp713I, Bsp63I, CstI, Ecl37kI, HalII, PaePI, PstI, YenI

MhlI Micrococcus halobius SD 5' GDGCHC
3' CHCGDG 5' ---GDGCH C--- 3'
3' ---C HCGDG--- 5' AocII, BmyI, BsoCI, Bsp1286I, BspLS2I, MhlI, SduI

MkrAI Micrococcus kristinae 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' Bme12I, BspAI, BstENII, ChaI, FnuEI, MgoI, NphI, Sau3AI

MlaI Mastigocladus laminosus 5' TTCGAA
3' AAGCTT 5' ---TT CGAA--- 3'
3' ---AAGC TT--- 5' Asp10HI, BimI, BsiCI, BstBI, Csp68KII, LspI, PlaII, Ssp1I

MlaAI Micromonospora lacustris 5' CTCGAG
3' GAGCTC 5' ---C TCGAG--- 3'
3' ---GAGCT C--- 5' BspAAI, BstVI, PanI, SauLPII, Sbi68I, Sfr274I, SlaI, XhoI

MlsI Micrococcus luteus Ng 16-122 5' TGGCCA
3' ACCGGT 5' ---TGG CCA--- 3'
3' ---ACC GGT--- 5'

MltI Micrococcus luteus 5' AGCT
3' TCGA 5' ---AG CT--- 3'
3' ---TC GA--- 5' AluBI, AluI

MluI Micrococcus luteus 5' ACGCGT
3' TGCGCA 5' ---A CGCGT--- 3'
3' ---TGCGC A--- 5'

Mlu23I Micrococcus luteus 23 5' GGATCC
3' CCTAGG 5' ---G GATCC--- 3'
3' ---CCTAG G--- 5'

Mlu31I Micrococcus luteus 31 5' TGGCCA
3' ACCGGT 5' ---TGG CCA--- 3'
3' ---ACC GGT--- 5'

MluB2I Micrococcus luteus B2 5' TCGCGA
3' AGCGCT 5' ---TCG CGA--- 3'
3' ---AGC GCT--- 5'

MluNI Micrococcus luteus N 5' TGGCCA
3' ACCGGT 5' ---TGG CCA--- 3'
3' ---ACC GGT--- 5'

MlyI Micrococcus lylae 5' GAGTC
3' CTCAG 5' ---GAGTCN₄N --- 3'
3' ---CTCAGN₄N --- 5'

Mly113I Micrococcus lylae 113 5' GGCGCC
3' CCGCGG 5' ---GG CGCC--- 3'
3' ---CCGC GG--- 5'

MmeI Methylophilus methylotrophus 5' TCCRAC
3' AGGYTG 5' ---TCCRACN₁₇NNN --- 3'
3' ---AGGYTGN₁₇N NN--- 5'

MnlI Moraxella nonliquefaciens 5' CCTC
3' GGAG 5' ---CCTCN₄NNN --- 3'
3' ---GGAGN₄NN N--- 5'

MnoI Moraxella nonliquefaciens 5' CCGG
3' GGCC 5' ---C CGG--- 3'
3' ---GGC C--- 5'

Mph1103I Moraxella phenylpyruvica RFL1103 5' ATGCAT
3' TACGTA 5' ---ATGCA T--- 3'
3' ---T ACGTA--- 5' BfrBI, Csp68KIII, EcoT22I, NsiI, PinBI, Ppu10I, SepI, SspD5II

MroI Micrococcus roseus 5' TCCGGA
3' AGGCCT 5' ---T CCGGA--- 3'
3' ---AGGCC T--- 5' BbvAIII, BseAI, Bsp13I, BspEI, Bsu23I, CauB3I, PinBII, PtaI

MroNI Micrococcus roseus N 5' GCCGGC
3' CGGCCG 5' ---G CCGGC--- 3'
3' ---CGGCC G--- 5'

MroXI Micrococcus roseus XM 5' GAAN₄TTC
3' CTTN₄AAG 5' ---GAANN NNTTC--- 3'
3' ---CTTNN NNAAG--- 5' Asp700I, BbvAI, PdmI, XmnI

MscI Micrococcus sp. 5' TGGCCA
3' ACCGGT 5' ---TGG CCA--- 3'
3' ---ACC GGT--- 5'

MseI Micrococcus sp. 5' TTAA
3' AATT 5' ---T TAA--- 3'
3' ---AAT T--- 5'

MslI Moraxella osloensis 5' CAYN₄RTG
3' GTRN₄YAC 5' ---CAYNN NNRTG--- 3'
3' ---GTRNN NNYAC--- 5'

MspI 1SA3, 1YFI, Search PDBe Moraxella sp. 5' CCGG
3' GGCC 5' ---C CGG--- 3'
3' ---GGC C--- 5'

Msp17I Micrococcus sp. 17 5' GRCGYC
3' CYGCRG 5' ---GR CGYC--- 3'
3' ---CYGC RG--- 5' AsuIII, BsaHI, BstACI, HgiGI, HgiHII, Hin1I, Msp17I, PamII

Msp20I Micrococcus sp. 5' TGGCCA
3' ACCGGT 5' ---TGG CCA--- 3'
3' ---ACC GGT--- 5'

Msp67I Micrococcus sp. MS67 5' CCNGG
3' GGNCC 5' ---CC NGG--- 3'
3' ---GGN CC--- 5'

MspA1I Moraxella sp. A1 5' CMGCKG
3' GKCGMC 5' ---CMG CKG--- 3'
3' ---GKC GMC--- 5'

MspB4I Moraxella sp. B4 5' GGYRCC
3' CCRYGG 5' ---G GYRCC--- 3'
3' ---CCRYG G--- 5' AccB1I, BanI, BshNI, BspT107I, Eco64I, HgiCI, HgiHI, PfaAI

MspCI Micrococcus sp. 5' CTTAAG
3' GAATTC 5' ---C TTAAG--- 3'
3' ---GAATT C--- 5'

MspR9I Micrococcus sp. R9 5' CCNGG
3' GGNCC 5' ---CC NGG--- 3'
3' ---GGN CC--- 5'

MspSWI Moraxella sp. SW 5' ATTTAAAT
3' TAAATTTA 5' ---ATTT AAAT--- 3'
3' ---TAAA TTTA--- 5'

MspV281I

Myxosarcinia sp. V/281

5' GWGCWC
3' CWCGWG

5' ---GWGCW C--- 3'
3' ---C WCGWG--- 5'

Alw21I, AspHI, Bbv12I, Bsh45I, BsiHKAI,
Bsm6I,	HgiAI,	HpyF46II,

MspYI Microcystis sp. J2 5' YACGTR
3' RTGCAY 5' ---YAC GTR--- 3'
3' ---RTG CAY--- 5'

MssI Methylobacterium sp. Dd 5-732 5' GTTTAAAC
3' CAAATTTG 5' ---GTTT AAAC--- 3'
3' ---CAAA TTTG--- 5' PmeI

MstI Microcoleus sp. 5' TGCGCA
3' ACGCGT 5' ---TGC GCA--- 3'
3' ---ACG CGT--- 5' Acc16I, AosI, AviII, FdiII, FspI, NsbI, PamI, Pun14627I

MstII Microcoleus sp. 5' CCTNAGG
3' GGANTCC 5' ---CC TNAGG--- 3'
3' ---GGANT CC--- 5' AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI

MthZI Methanobacterium thermoformicicum Z-245 5' CTAG
3' GATC 5' ---C TAG--- 3'
3' ---GAT C--- 5'

MunI 1D02 Mycoplasma sp. 5' CAATTG
3' GTTAAC 5' ---C AATTG--- 3'
3' ---GTTAA C--- 5'

MvaI 2OAA, 2OA9, Search PDBe, 2OA9 Micrococcus varians RFL19 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' AjnI, BciBII, BptI, Bst1I, BstOI, BstM6I, Bst2UI, SslI, TaqXI, ZanI

Mva1269I Micrococcus varians RFL1269 5' GAATGC
3' CTTACG 5' ---GAATGCN --- 3'
3' ---CTTAC GN--- 5' Asp26HI, Asp40HI, Asp50HI, BscCI, BsmI, Mva1269I, PctI

MvnI Methanococcus vannielii 5' CGCG
3' GCGC 5' ---CG CG--- 3'
3' ---GC GC--- 5' AccII, Bsh1236I, BstFNI, BtkI, Csp68KVI, FalII, FauBII, ThaI

MvrI Micrococcus varians 5' CGATCG
3' GCTAGC 5' ---CGAT CG--- 3'
3' ---GC TAGC--- 5' Afa22MI, BspCI, EagBI, ErhB9I, Ple19I, PvuI, RshI, XorII

MwoI Methanobacterium wolfei 5' GCN₇GC
3' CGN₇CG 5' ---GCNNNNN NNGC--- 3'
3' ---CGNN NNNNNCG--- 5'

MxaI Myxococcus xanthus F18E 5' GAGCTC
3' CTCGAG 5' ---GAG CTC--- 3'
3' ---CTC GAG--- 5'

N

Enzyme PDB code Source Recognition sequence Cut Isoschizomers

NaeI 1EV7, 1iaw, Search PDB Nocardia aerocolonigenes 5' GCCGGC
3' CGGCCG 5' ---GCC GGC--- 3'
3' ---CGG CCG--- 5'

NarI Nocardia argentinensis 5' GGCGCC
3' CCGCGG 5' ---GG CGCC--- 3'
3' ---CCGC GG--- 5'

NblI Nocardia blackwellii 5' CGATCG
3' GCTAGC 5' ---CGAT CG--- 3'
3' ---GC TAGC--- 5' Afa16RI, Afa22MI, EagBI, MvrI, Ple19I, Psu161I, RshI, XorII

NciI

Neisseria cinerea

5' CCSGG
3' GGSCC

5' ---CC SGG--- 3'
3' ---GGS CC--- 5'

AhaI, AseII, AsuC2I,	BpuMI,
CauII, EcoHI, HgiS22I, Mgl14481I

NcoI Nocardia corallina 5' CCATGG
3' GGTACC 5' ---C CATGG--- 3'
3' ---GGTAC C--- 5'

NcrI Nocardia carnea C-212 5' AGATCT
3' TCTAGA 5' ---A GATCT--- 3'
3' ---TCTAG A--- 5'

NcuI Neisseria cuniculi 5' GAAGA
3' CTTCT 5' ---GAAGAN₆NN --- 3'
3' ---CTTCTN₆N N--- 5'

NdaI Nocardia dassonvillei 5' GGCGCC
3' CCGCGG 5' ---GG CGCC--- 3'
3' ---CCGC GG--- 5'

NdeI Neisseria denitrificans 5' CATATG
3' GTATAC 5' ---CA TATG--- 3'
3' ---GTAT AC--- 5'

NdeII Neisseria denitrificans 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' Bce243I, Bsp105I, BspJI, BstMBI, CviAI, Kzo9I, NlaII, Sth368I

NgoAIII Neisseria gonorrhoeae FA1090 5' CCGCGG
3' GGCGCC 5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'

NgoAIV Neisseria gonorrhoeae FA1090 5' GCCGGC
3' CGGCCG 5' ---G CCGGC--- 3'
3' ---CGGCC G--- 5'

NgoMIV 1FIU, 4abt Search PDBe θ Neisseria gonorrhoeae MS11 5' GCCGGC
3' CGGCCG 5' ---G CCGGC--- 3'
3' ---CGGCC G--- 5'

NgoPII Neisseria gonorrhoeae P9-2 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

NgoPIII Neisseria gonorrhoeae P9-2 5' CCGCGG
3' GGCGCC 5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'

NheI

Neisseria mucosa heidelbergensis

5' GCTAGC
3' CGATCG

5' ---G CTAGC--- 3'
3' ---CGATC G--- 5'

AceII, AsuNHI, BmtI,	BspOI,
LlaG2I, PstNHI

NlaII Neisseria lactamica 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' BscFI, BspAI, BstENII, ChaI, FnuEI, MgoI, RalF40I, Sau3AI

NlaIII Neisseria lactamica 5' CATG
3' GTAC 5' ---CATG --- 3'
3' --- GTAC--- 5'

NlaIV

Neisseria lactamica

5' GGNNCC
3' CCNNGG

5' ---GGN NCC--- 3'
3' ---CCN NGG--- 5'

AspNI, BscBI,	BmiI,	BspLI,
PspN4I

Nli3877I Nostoc linckia 5' CYCGRG
3' GRGCYC 5' ---CYCGR G--- 3'
3' ---G RGCYC--- 5' Ama87I, AvaI, BcoI, BsiHKCI, Eco27kI, Eco88I, NspSAI, OfoI

NmeCI Neisseria meningitidis C114 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' BscFI, BspAI, BstENII, ChaI, FnuEI, MgoI, RalF40I, Sau3AI

NmeRI Neisseria meningitidis 5' CAGCTG
3' GTCGAC 5' ---CAG CTG--- 3'
3' ---GTC GAC--- 5'

NmuCI Neisseria mucosa C9-2 5' GTSAC
3' CASTG 5' --- GTSAC--- 3'
3' ---CASTG --- 5'

NopI Nocardia opaca 5' GTCGAC
3' CAGCTG 5' ---G TCGAC--- 3'
3' ---CAGCT G--- 5'

NotI 3C25, 3BVQ, Search PDBe Nocardia otitidis-caviarum 5' GCGGCCGC
3' CGCCGGCG 5' ---GC GGCCGC--- 3'
3' ---CGCCGG CG--- 5'

NphI Neisseria pharyngis C245 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' AspMDI, BspAI, BstENII, ChaI, FnuEI, MkrAI, RalF40I, Sau3AI

NruI Nocardia rubra 5' TCGCGA
3' AGCGCT 5' ---TCG CGA--- 3'
3' ---AGC GCT--- 5'

NruGI

Nocardia sp. G

5' GACN₅GTC
3' CTGN₅CAG

5' ---GACNNN NNGTC--- 3'
3' ---CTGNN NNNCAG--- 5'

AhdI, AspEI,	BmeRI,	BspOVI,
DriI, Eam1105I, EclHKI

NsbI Neisseria subflava Va-1 5' TGCGCA
3' ACGCGT 5' ---TGC GCA--- 3'
3' ---ACG CGT--- 5' Acc16I, AosI, AviII, FdiII, FspI, MstI, PamI, Pun14627I

NsiI Neisseria sicca 5' ATGCAT
3' TACGTA 5' ---ATGCA T--- 3'
3' ---T ACGTA--- 5' BfrBI, Csp68KIII, EcoT22I, PinBI, Ppu10I, SepI, SspD5II, Zsp2I

NsiCI Neisseria sicca C351 5' GATATC
3' CTATAG 5' ---GAT ATC--- 3'
3' ---CTA TAG--- 5'

NspI Nostoc sp. C 5' RCATGY
3' YGTACR 5' ---RCATG Y--- 3'
3' ---Y GTACR--- 5'

NspII Nostoc sp. C 5' GDGCHC
3' CHCGDG 5' ---GDGCH C--- 3'
3' ---C HCGDG--- 5' AocII, BmyI, BsoCI, Bsp1286I, MhlI, NspII, SduI

NspIII Nostoc sp. C 5' CYCGRG
3' GRGCYC 5' ---C YCGRG--- 3'
3' ---GRGCY C--- 5' Ama87I, AvaI, Bse15I, BsoBI, Eco27kI, Eco88I, OfoI, PunAI

NspIV Nostoc sp. C 5' GGNCC
3' CCNGG 5' ---G GNCC--- 3'
3' ---CCNG G--- 5' AvcI, Bal228I, Bce22I, BsiZI, BspF4I, Cfr13I, Nsp7121I, UnbI

NspV Nostoc sp. C 5' TTCGAA
3' AAGCTT 5' ---TT CGAA--- 3'
3' ---AAGC TT--- 5' Asp10HI, BimI, Bsp119I, BstBI, Csp68KII, MlaI, PlaII, SspRFI

Nsp7121I Nostoc sp. 7121 5' GGNCC
3' CCNGG 5' ---G GNCC--- 3'
3' ---CCNG G--- 5' AvcI, Bal228I, Bce22I, Bsp1894I, Bsu54I, Cfr13I, NspIV, UnbI

Nsp29132II Nostoc sp. 5' GGATCC
3' CCTAGG 5' ---G GATCC--- 3'
3' ---CCTAG G--- 5' AliI, ApaCI, BamHI, Bce751I, Nsp29132II, Pfl8I, SolI, Uba4009I

NspBII Nostoc sp. 5' CMGCKG
3' GKCGMC 5' ---CMG CKG--- 3'
3' ---GKC GMC--- 5'

NspHI Nostoc sp. 5' RCATGY
3' YGTACR 5' ---RCATG Y--- 3'
3' ---Y GTACR--- 5'

NspLKI Nocardia sp. LK 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

NspMACI Nostoc sp. 5' AGATCT
3' TCTAGA 5' ---A GATCT--- 3'
3' ---TCTAG A--- 5'

NspSAI Nostoc sp. SA 5' CYCGRG
3' GRGCYC 5' ---C YCGRG--- 3'
3' ---GRGCY C--- 5' Ama87I, AvaI, Bse15I, BspLU4I, Eco27kI, Nli3877I, OfoI, PunAI

NspSAII Nostoc sp. SA 5' GGTNACC
3' CCANTGG 5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5' AspAI, Bse64I, BseT9I, BseT10I, BstPI, Eco91I, NspSAII, PspEI

NspSAIV Nostoc sp. SA 5' GGATCC
3' CCTAGG 5' ---G GATCC--- 3'
3' ---CCTAG G--- 5' AliI, BamHI, Bsp98I, Bsp4009I, NspSAIV, Pfl8I, SolI, Uba4009I

NunII Nocardia uniformis 5' GGCGCC
3' CCGCGG 5' ---GG CGCC--- 3'
3' ---CCGC GG--- 5'

v t e Restriction modification system: restriction enzyme
Basic Concept	Isoschizomer Neoschizomer Isocaudomer
Recognition sequence 4bp	TaqI Sau3A AluI* HaeIII* DpnII NlaIII HpaII
Recognition sequence 5bp	EcoRII FokI
Recognition sequence 6bp	AaaI BglII PovII* EcoRV* EcoRI BamHI HindIII XbaI XhoI SacI PstI NdeI
Recognition sequence 8bp	NotI
Lists	A Ba–Bc Bd–Bp Bsa–Bso Bsp–Bss Bst–Bv C-D E-F G-K L-N O-R S T-Z
* means cleavage produces blunt ends