C12orf42

C12orf42
Identifiers
Aliases	C12orf42, chromosome 12 open reading frame 42
External IDs	MGI: 1923890 HomoloGene: 45695 GeneCards: C12orf42
Gene location (Human) Chr. Chromosome 12 (human) Band 12q23.2-q23.3 Start 103,237,591 bp End 103,495,978 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in sperm spleen lymph node appendix blood bone marrow cells endothelial cell thymus sural nerve prefrontal cortex n/a More reference expression data BioGPS n/a
Orthologs Species Human Mouse Entrez 374470 76640 Ensembl ENSG00000179088 n/a UniProt Q96LP6 n/a RefSeq (mRNA) NM_001099336 NM_001278419 NM_001278420 NM_198521 NM_001386867 NM_001386868 NM_001386869 NM_001386871 NM_001386872 NM_029685 RefSeq (protein) NP_001092806 NP_001265348 NP_001265349 NP_940923 n/a Location (UCSC) Chr 12: 103.24 – 103.5 Mb n/a PubMed search
Wikidata
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Chromosome 12 Open Reading Frame 42 (C12orf42) is a protein-encoding gene in Homo sapiens.

Gene

Locus

The genomic location for this gene is as follows: starts at 103,237,591 bp and ends 103,496,010 bp. The cytogenetic location for C12orf42 is 12q23.2. It is located on the negative strand

Cytogenetic band: 12q23.2

mRNA

Fifteen different mRNAs are made by transcription: fourteen alternative splice variants and one unspliced form.

Protein

Tertiary structure for C12orf42 protein.

The protein released by this gene is known as uncharacterized protein C12orf42. There are three isoforms for this protein produced by alternative splicing. The first isoform is a conical sequence. The second Isoform differs form the first in that it doesn't contain 1-95 aa in its sequence. The third isoform differs from the conical sequence in two ways:

• 87-107 aa is the sequence GSHHGQATQKLQGAMVLHLEE instead of VFPERTQNSMACKRLLHTCQY
• the entire sequence 108-360 aa doesn't exist in this isoform

Secondary structure

C12orf42 protein takes on several secondary structures, such as: alpha helices, beta sheets, and random coils. C12orf42 protein is a soluble. Proteins that are soluble have a hydrophilic outside and hydrophobic interior . Proteins with this type of structure are able to freely float inside a cell, due to the liquid composition of the cytosol.

Subcellular location

C12orf42 is an intracellular protein. This is known by the lack of transmembrane domains or signal peptides. This suggests that it is predicted to be a nuclear protein, given the nuclear localization signal (NSL) found: PRDRRPQ at 292 aa and a bipartite KRLIKVCSSAPPRPTRR at 325 aa.

This protein cartoon illustrates the location of, the domain DUF4607 and two nuclear localization sequences.

Post-translation modification

Predicted post-translation modification sites are seen below in the table. Nuclear proteins are known for having phosphorylation, acetylation, sumoylation, and O-GlcNAc as types of modifications:

• Phosphorylation affects proteins-protein interaction and the stability of the protein.
• Acetylation promotes protein folding and improves stability.
• Sumoylation is involved in nuclear-cytosolic transport and DNA repair.
• Glycosylation (known as O-GlcNAc while in the nucleus) promotes protein folding and stability.

Expression

Tissue profiles

Microarray data shows expression of the C12orf42 gene in different tissues throughout the human body. There is high expression in the lymph node, spleen, and thymus. There is significant expression in the brain, bladder, epididymis, and the helper T cell. Therefore, there is statistically significant expression of C12orf42 gene throughout the nervous system, immune system, and male reproductive system.

In situ hybridization

The table below shows the areas in the mouse brain where C12orf42 is expressed. The gene name for the mouse is 1700113H08Rik, it is the human homolog of C12orf42. Area one and two of the brain manages body and skeletal movement. Areas three and four in the brain are for sensory functions; area four specializes in perception of smell. Area five in the brain functions in emotional learning and memory.

Homology

Paralog

C12orf42 gene has only one other member in its gene family, this gene is known as Neuroligin 4, Y linked gene (NLGN4Y).

Orthologs

C12orf42 orthologs are mostly mammals. One exception that was found is the Pelodiscus Sinensis or more commonly known as the Chinese soft-shell turtle.

Conserved domain structure

The domain structure that is most important is DUF4607, it is conserved in the Eutheria clade in the Mammalia class. The order that it is conserved in is as follows: Artiodactyla, Carnivora, Chiroptera, Lagomorpha, Perissodactyla, Primates, Proboscidea, and Rodentia.

Clinical significance

In an experiment, fine-tiling comparative genomic hybridization (FT-CGH) and ligation-mediated PCR (LM-PCR) were combined. This resulted in the finding of a chromosomal translocation t(12;14)(q23;q11.2) in  T-lymphoblastic lymphoma (T-LBL). The chromosomal translocation occurs during T-receptor delta gene-deleting rearrangement, which is important in T-cell differentiation. This translocation disrupts C12orf42 and it brings the gene ASCL1 closer to the T-cell receptor alpha (TRA) enhancer. As a result, the cross-fused gene encodes vital transcription factors that are found in medullary thyroid cancer and small-cell lung cancer.

Illustrates the translocation that occurred that led to a deletion in chromosome 12. Where chromosome 12q23 cross-fused with TRDREC and TRAJ61 segment. This interfered with C12orf42 gene.