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List of restriction enzyme cutting sites: Bd–Bp
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    List of restriction enzyme cutting sites: Bd–Bp

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    Legend of nucleobases
    Code Nucleotide represented
    A Adenine (A)
    C Cytosine (C)
    G Guanine (G)
    T Thymine (T)
    N A, C, G or T
    M A or C
    R A or G
    W A or T
    Y C or T
    S C or G
    K G or T
    H A, C or T
    B C, G or T
    V A, C or G
    D A, G or T

    This article contains a list of the most studied restriction enzymes whose names start with Bd to Bp inclusive. It contains approximately 100 enzymes.

    The following information is given:

    • Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature, and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
    • PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action.
    • Source: Organism that naturally produces the enzyme.
    • Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
    • Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site.
    • Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:
    as in this not listed enzyme:  EcoR70I 


    Whole list navigation

    Restriction enzymes

    Bd - Bp

    Enzyme PDB code Source Recognition sequence Cut Isoschizomers
    BdiI Brevibacterium divaricatum 5' ATCGAT
    3' TAGCTA
    5' ---AT   CGAT--- 3'
    3' ---TAGC   TA--- 5'
    BavCI, Bci29I, Bli41I, Bli86I, BseCI, BsuTUI, Rme21I, SpmI
    BdiSI Bacteroides distasonis 5' CTRYAG
    3' GAYRTC
    5' ---C   TRYAG--- 3'
    3' ---GAYRT   C--- 5'
    BecAII Brevibacterium sp. A 5' GGCC
    3' CCGG
    5' ---GG   CC--- 3'
    3' ---CC   GG--- 5'
    BepI Brevibacterium epidermidis 5' CGCG
    3' GCGC
    5' ---CG   CG--- 3'
    3' ---GC   GC--- 5'
    AccII, BepI, BspFNI, BstUI, Bsu1532I, BtkI, FalII, FauBII, ThaI
    BetI Brevibacterium acetyliticum 5' WCCGGW
    3' WGGCCW
    5' ---W   CCGGW--- 3'
    3' ---WGGCC   W--- 5'
    BfaI Bacteroides fragilis 5' CTAG
    3' GATC
    5' ---C   TAG--- 3'
    3' ---GAT   C--- 5'
    BfiI 2C1L Bacillus firmus S8120 5' ACTGGG
    3' TGACCC
    5' ---ACTGGGNNNNN   --- 3'
    3' ---TGACCCNNNN   N--- 5'
    BmrI
    Bfi57I Butyrivibrio fibrisolvens OB157 5' GATC
    3' CTAG
    5' ---   GATC--- 3'
    3' ---CTAG   --- 5'
    Bsp105I, Bsp143I, BspJI, BstMBI, CviAI, Kzo9I, NdeII, SsiBI
    Bfi89I Butyrivibrio fibrisolvens OB189 5' YGGCCR
    3' RCCGGY
    5' ---Y   GGCCR--- 3'
    3' ---RCCGG   Y--- 5'
    BflI Bacillus flavothermus 5' CCN7GG
    3' GGN7CC
    5' ---CCNNNNN   NNGG--- 3'
    3' ---GGNN   NNNNNCC--- 5'
    BfmI Bacillus firmus S8-336 5' CTRYAG
    3' GAYRTC
    5' ---C   TRYAG--- 3'
    3' ---GAYRT   C--- 5'
    BfrI Bacteroides fragilis 5' CTTAAG
    3' GAATTC
    5' ---C   TTAAG--- 3'
    3' ---GAATT   C--- 5'
    BfrBI Bacteroides fragilis BE3 5' ATGCAT
    3' TACGTA
    5' ---ATGCA   T--- 3'
    3' ---T   ACGTA--- 5'
    Csp68KIII, EcoT22I, Mph1103I, PinBI, Ppu10I, SepI, SspD5II, Zsp2I
    BfuI Bacillus firmus Auk 22-m21 5' GTATCC
    3' CATAGG
    5' ---GTATCCN4NN   --- 3'
    3' ---CATAGGN4N   N--- 5'
    BciVI
    BfuAI Bacillus fusiformis 5' ACCTGC
    3' TGGACG
    5' ---ACCTGCNNNN   NNNN--- 3'
    3' ---TGGACGNNNNNNNN   --- 5'
    Acc36I, BspMI, BveI
    BfuCI Bacillus fusiformis 1226 5' GATC
    3' CTAG
    5' ---   GATC--- 3'
    3' ---CTAG   --- 5'
    Bce243I, Bsp105I, BspFI, BstKTI, CpfI, HacI, MkrAI, Sth368I
    BglI 1DMU Bacillus globigii 5' GCCN5GGC
    3' CGGN5CCG
    5' ---GCCNNNN   NGGC--- 3'
    3' ---CGGN   NNNNCCG--- 5'
    BglII 1ES8 Bacillus globigii 5' AGATCT
    3' TCTAGA
    5' ---A   GATCT--- 3'
    3' ---TCTAG   A--- 5'
    BimI Brevibacterium immotum 5' TTCGAA
    3' AAGCTT
    5' ---TT   CGAA--- 3'
    3' ---AAGC   TT--- 5'
    AcpI, AsuII, Bpu14I, BspT104I, CbiI, FspII, MlaI, PpaAI, SfuI
    Bim19I Brevibacterium immotum 19 5' TTCGAA
    3' AAGCTT
    5' ---TT   CGAA--- 3'
    3' ---AAGC   TT--- 5'
    AcpI, AsuII, Bpu14I, BspT104I, CbiI, FspII, MlaI, PpaAI, SfuI
    Bim19II Brevibacterium immotum 19 5' GGCC
    3' CCGG
    5' ---GG   CC--- 3'
    3' ---CC   GG--- 5'
    BinI Bifidobacterium infantis 659 5' GGATC
    3' CCTAG
    5' ---GGATCNNNN   N--- 3'
    3' ---CCTAGNNNNN   --- 5'
    AclWI, AlwI,  BsrWI,  BspPI,
    BstH9I,  Bth617I,  EacI
    BlfI Bacillus licheniformis 5' TCCGGA
    3' AGGCCT
    5' ---T   CCGGA--- 3'
    3' ---AGGCC   T--- 5'
    AccIII, Aor13HI, BseAI, BsiMI, BspMII, Bsu23I, Kpn2I, PinBII
    Bli41I Bacillus licheniformis 41 5' ATCGAT
    3' TAGCTA
    5' ---AT   CGAT--- 3'
    3' ---TAGC   TA--- 5'
    Bci29I, Bli86I, BseCI, BsiXI, BsuTUI, Rme21I, SpmI, Ssp27144I
    Bli86I Bacillus licheniformis 86 5' ATCGAT
    3' TAGCTA
    5' ---AT   CGAT--- 3'
    3' ---TAGC   TA--- 5'
    BliAI, Bli41I, BseDI, BsiXI, Rme21I, SpmI, Ssp27144I, ZhoI
    Bli736I Bacillus licheniformis 736 5' GGTCTC
    3' CCAGAG
    5' ---GGTCTCN   NNNN--- 3'
    3' ---CCAGAGNNNNN   --- 5'
    BliAI Bacillus licheniformis 5' ATCGAT
    3' TAGCTA
    5' ---AT   CGAT--- 3'
    3' ---TAGC   TA--- 5'
    BliRI, Bli41I, BsiXI, BspDI, Rme21I, SpmI, Ssp27144I, ZhoI
    BliHKI Bacillus licheniformis HK 5' CCTNAGG
    3' GGANTCC
    5' ---CC   TNAGG--- 3'
    3' ---GGANT   CC--- 5'
    AocI, AxyI, Bse21I, Bsu36I, Eco81I, MstII, SauI, SshAI
    BliRI Bacillus licheniformis 5' ATCGAT
    3' TAGCTA
    5' ---AT   CGAT--- 3'
    3' ---TAGC   TA--- 5'
    BciBI, BdiI, Bli41I, BsiXI, BspDI, BspXI, LplI, SpmI, ZhoI
    BlnI Brevibacterium linens 5' CCTAGG
    3' GGATCC
    5' ---C   CTAGG--- 3'
    3' ---GGATC   C--- 5'
    AspA2I, AvrII, AvrBII, BspA2I, XmaJI
    BloHI Bifidobacterium longum E194b 5' RGATCY
    3' YCTAGR
    5' ---R   GATCY--- 3'
    3' ---YCTAG   R--- 5'
    BloHII Bifidobacterium longum E194b 5' CTGCAG
    3' GACGTC
    5' ---CTGCA   G--- 3'
    3' ---G   ACGTC--- 5'
    AjoI, Asp713I, BsuBI, CflI, CfuII, HalII, PstI, SalPI, SflI, Sst12I
    BlpI Bacillus lentus 5' GCTNAGC
    3' CGANTCG
    5' ---GC   TNAGC--- 3'
    3' ---CGANT   CG--- 5'
    BluI Brevibacterium luteum 5' CTCGAG
    3' GAGCTC
    5' ---C   TCGAG--- 3'
    3' ---GAGCT   C--- 5'
    AbrI, BssHI, BstVI, PanI, Sau3239I, Sfr274I, TliI, XhoI
    Bme12I Bacillus megaterium 12 5' GATC
    3' CTAG
    5' ---   GATC--- 3'
    3' ---CTAG   --- 5'
    Bfi57I, Bsp143I, BspJI, BstMBI, CviAI, Kzo9I, NlaII, SsiBI
    Bme18I Bacillus megaterium 18 5' GGWCC
    3' CCWGG
    5' ---G   GWCC--- 3'
    3' ---CCWG   G--- 5'
    BamNxI, BcuAI, Csp68KI, EagMI, FssI, HgiCII, HgiJI, SinI
    Bme142I Bacillus megaterium 142 5' RGCGCY
    3' YCGCGR
    5' ---RGC   GCY--- 3'
    3' ---YCG   CGR--- 5'
    AccB2I,  BfoI,  Bsp143II,
    BstH2I, HaeII, LpnI
    Bme216I Bacillus megaterium 216 5' GGWCC
    3' CCWGG
    5' ---G   GWCC--- 3'
    3' ---CCWG   G--- 5'
    BamNxI, BcuAI, Csp68KI, Eco47I, FssI, HgiCII, HgiJI, SinI
    Bme361I Bacillus megaterium 361 5' GGCC
    3' CCGG
    5' ---GG   CC--- 3'
    3' ---CC   GG--- 5'
    Bme585I Bacillus mesentericus 585 5' CCCGC
    3' GGGCG
    5' ---CCCGCNNNN   NN--- 3'
    3' ---GGGCGNNNNNN   --- 5'
    Bme1390I Bacillus megaterium RFL1390 5' CCNGG
    3' GGNCC
    5' ---CC   NGG--- 3'
    3' ---GGN   CC--- 5'
    Bme1580I Bacillus megaterium 1580 5' GKGCMC
    3' CMCGKG
    5' ---GKGCM   C--- 3'
    3' ---C   MCGKG--- 5'
    BmgBI Bacillus megaterium 5' CACGTC
    3' GTGCAG
    5' ---CAC   GTC--- 3'
    3' ---GTG   CAG--- 5'
    BmrI Bacillus megaterium 5' ACTGGG
    3' TGACCC
    5' ---ACTGGGNNNNN   --- 3'
    3' ---TGACCCNNNN   N--- 5'
    BfiI
    BmtI Bacillus megaterium S2 5' GCTAGC
    3' CGATCG
    5' ---GCTAG   C--- 3'
    3' ---C   GATCG--- 5'
    AceII, AsuNHI,  BspOI,  NheI,
    LlaG2I, PstNHI
    BmyI Bacillus mycoides 5' GDGCHC
    3' CHCGDG
    5' ---GDGCH   C--- 3'
    3' ---C   HCGDG--- 5'
    AocII, BsoCI, Bsp1286I, BspLS2I, MhlI, NspII, SduI
    BnaI Bacillus natto B3364 5' GGATCC
    3' CCTAGG
    5' ---G   GATCC--- 3'
    3' ---CCTAG   G--- 5'
    AccEBI, BamHI, Bce751I, BstI, CelI, OkrAI, Nsp29132II, Pfl8I, SolI
    BoxI Brevibacterium oxydans Iti 12-025 5' GACN4GTC
    3' CTGN4CAG
    5' ---GACNN   NNGTC--- 3'
    3' ---CTGNN   NNCAG--- 5'
    BpcI Bacillus sphaericus 5' CTRYAG
    3' GAYRTC
    5' ---C   TRYAG--- 3'
    3' ---GAYRT   C--- 5'
    BpiI Bacillus pumilus sw 4-3 5' GAAGAC
    3' CTTCTG
    5' ---GAAGACNN   NNNN--- 3'
    3' ---CTTCTGNNNNNN   --- 5'
    BplI Bacillus pumilus 5' GAGN5CTC
    3' CTCN5GAG
    5' ---GAGN5CTCN7NNNNNN   --- 3'
    3' ---CTCN5GAGN7N   NNNNN--- 5'
    BpmI Bacillus pumilus 5' CTGGAG
    3' GACCTC
    5' ---CTGGAGN13NNN   --- 3'
    3' ---GACCTCN13N   NN--- 5'
    BpoAI Bacillus polymyxa 5' ATTAAT
    3' TAATTA
    5' ---AT   TAAT--- 3'
    3' ---TAAT   TA--- 5'
    AseI, AsnI, PshBI, Sru4DI, VspI
    BptI Bacillus pantothenticus 5' CCWGG
    3' GGWCC
    5' ---CC   WGG--- 3'
    3' ---GGW   CC--- 5'
    AjnI, BseBI, Bse16I, BstNI, BstOI, BstM6I, Bst2UI, EcoRII, MvaI
    BpuI Bacillus pumilus AHU1387A 5' GRGCYC
    3' CYCGRG
    5' ---GRGCY   C--- 3'
    3' ---C   YCGRG--- 5'
    Bpu10I Bacillus pumilus 10 5' CCTNAGC
    3' GGANTCG
    5' ---CC   TNAGC--- 3'
    3' ---GGANT   CG--- 5'
    Bpu14I Bacillus pumilus 14 5' TTCGAA
    3' AAGCTT
    5' ---TT   CGAA--- 3'
    3' ---AAGC   TT--- 5'
    AcpI, AsuII, BsiCI, BspT104I, CbiI, FspII, MlaI, PpaAI, SfuI
    Bpu95I Bacillus pumilus 95 5' CGCG
    3' GCGC
    5' ---CG   CG--- 3'
    3' ---GC   GC--- 5'
    AccII, BepI, BspFNI, BstUI, Bsu1532I, FalII, FnuDII, MvnI, ThaI
    Bpu1102I Bacillus pumilus RFL1102 5' GCTNAGC
    3' CGANTCG
    5' ---GC   TNAGC--- 3'
    3' ---CGANT   CG--- 5'
    BpuAI Bacillus pumilus 5' GAAGAC
    3' CTTCTG
    5' ---GAAGACNN   NNNN--- 3'
    3' ---CTTCTGNNNNNN   --- 5'
    BpuAmI Bacillus pumilus 5' GAGCTC
    3' CTCGAG
    5' ---GAG   CTC--- 3'
    3' ---CTC   GAG--- 5'
    BpuB5I Bacillus pumilus 5' CGTACG
    3' GCATGC
    5' ---C   GTACG--- 3'
    3' ---GCATG   C--- 5'
    BpuDI Bacillus pumilus 1117 5' CCTNAGC
    3' GGANTCG
    5' ---CC   TNAGC--- 3'
    3' ---GGANT   CG--- 5'
    BpuEI Bacillus pumilus 2187a 5' CTTGAG
    3' GAACTC
    5' ---CTTGAGN13NNN   --- 3'
    3' ---GAACTCN13N   NN--- 5'
    BpuJI 2VLA Bacillus pumilus RFL1458 5' CCCGT
    3' GGGCA
    5' ---CCCGTN10N   NNNNNNNN--- 3'
    3' ---GGGCAN10NNNNNNNNN   --- 5'
     — None in May 2010 —
    BpuSI Bacillus pumilus 5' GGGAC
    3' CCCTG
    5' ---GGGACN9N   NNNN--- 3'
    3' ---CCCTGN9NNNNN   --- 5'

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