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List of restriction enzyme cutting sites: E–F
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Main articles: List of restriction enzyme cutting sites and Restriction enzyme
Legend of nucleobases | |
---|---|
Code | Nucleotide represented |
A | Adenine (A) |
C | Cytosine (C) |
G | Guanine (G) |
T | Thymine (T) |
N | A, C, G or T |
M | A or C |
R | A or G |
W | A or T |
Y | C or T |
S | C or G |
K | G or T |
H | A, C or T |
B | C, G or T |
V | A, C or G |
D | A, G or T |
This article contains a list of the most studied restriction enzymes whose names start with E to F inclusive. It contains approximately 110 enzymes.
The following information is given:
- Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature, and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
- PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action.
- Source: Organism that naturally produces the enzyme.
- Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
- Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site.
- Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:
as in this not listed enzyme: EcoR70I
Whole list navigation
Restriction enzymes
E
Enzyme | PDB code | Source | Recognition sequence | Cut | Isoschizomers | ||||||
EacI | Eubacterium acidaminophilum |
5' GGATC 3' CCTAG
|
5' ---GGATCNNNN N--- 3' 3' ---CCTAGNNNNN --- 5'
|
|
|||||||
EaeI | Enterobacter aerogenes |
5' YGGCCR 3' RCCGGY
|
5' ---Y GGCCR--- 3' 3' ---RCCGG Y--- 5'
|
||||||||
Eae46I | Enterobacter aerogenes 46 |
5' CCGCGG 3' GGCGCC
|
5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5'
|
||||||||
EaeAI | Enterobacter aerogenes |
5' CCCGGG 3' GGGCCC
|
5' ---C CCGGG--- 3' 3' ---GGGCC C--- 5'
|
AhyI, Cfr9I, EclRI, PaeBI, PspAI, PspALI, XcyI, XmaI, XmaCI | |||||||
EagI | Enterobacter agglomerans |
5' CGGCCG 3' GCCGGC
|
5' ---C GGCCG--- 3' 3' ---GCCGG C--- 5'
|
AaaI, BseX3I, BstZI, EclXI, Eco52I, SenPT16I, XmaIII | |||||||
EagBI | Enterobacter agglomerans CBNU45 |
5' CGATCG 3' GCTAGC
|
5' ---CGAT CG--- 3' 3' ---GC TAGC--- 5'
|
Afa16RI, Afa22MI, BspCI, ErhB9I, NblI, Psu161I, RshI, XorII | |||||||
EagMI | Enterobacter agglomerans M3 |
5' GGWCC 3' CCWGG
|
5' ---G GWCC--- 3' 3' ---CCWG G--- 5'
|
Bme18I, BsrAI, Csp68KI, Eco47I, FssI, HgiEI, Kzo49I, SmuEI | |||||||
Eam1104I | Enterobacter amnigenus RFL1104 |
5' CTCTTC 3' GAGAAG
|
5' ---CTCTTCN NNN--- 3' 3' ---GAGAAGNNNN --- 5'
|
||||||||
Eam1105I | Enterobacter amnigenus RFL1105 |
5' GACN5GTC 3' CTGN5CAG
|
5' ---GACNNN NNGTC--- 3' 3' ---CTGNN NNNCAG--- 5'
|
|
|||||||
EarI | Enterobacter aerogenes |
5' CTCTTC 3' GAGAAG
|
5' ---CTCTTCN NNN--- 3' 3' ---GAGAAGNNNN --- 5'
|
||||||||
EcaI | Enterobacter cloacae |
5' GGTNACC 3' CCANTGG
|
5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5'
|
AcrII, Bse64I, BseT10I, BstEII, Eco91I, EcoO65I, EcoO128I | |||||||
EciI | Escherichia coli |
5' GGCGGA 3' CCGCCT
|
5' ---GGCGGAN8NNN --- 3' 3' ---CCGCCTN8N NN--- 5'
|
||||||||
Eci125I | Escherichia coli VKM-125 |
5' GGTNACC 3' CCANTGG
|
5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5'
|
AcrII, AspAI, BseT10I, BstEII, Eco91I, EcoO65I, EcoO128I | |||||||
EclI | Enterobacter cloacae |
5' CAGCTG 3' GTCGAC
|
5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5'
|
||||||||
Ecl136II | Enterobacter cloacae RFL136 |
5' GAGCTC 3' CTCGAG
|
5' ---GAG CTC--- 3' 3' ---CTC GAG--- 5'
|
||||||||
EclHKI | Enterobacter cloacae |
5' GACN5GTC 3' CTGN5CAG
|
5' ---GACNNN NNGTC--- 3' 3' ---CTGNN NNNCAG--- 5'
|
|
|||||||
EclRI | Enterobacter cloacae R |
5' CCCGGG 3' GGGCCC
|
5' ---C CCGGG--- 3' 3' ---GGGCC C--- 5'
|
AhyI, Cfr9I, EaeAI, Pac25I, PspAI, PspALI, XcyI, XmaI, XmaCI | |||||||
EclXI | Enterobacter cloacae 590 |
5' CGGCCG 3' GCCGGC
|
5' ---C GGCCG--- 3' 3' ---GCCGG C--- 5'
|
AaaI, BseX3I, BstZI, EagI, Eco52I, SenPT16I, XmaIII | |||||||
Ecl18kI | 2FQZ | Enterobacter cloacae 18k |
5' CCNGG 3' GGNCC
|
5' --- CCNGG--- 3' 3' ---GGNCC --- 5'
|
|||||||
Ecl37kI | Enterobacter cloacae 37k |
5' CTGCAG 3' GACGTC
|
5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5'
|
AjoI, AliAJI, Ecl2zI, PaePI, Psp23I, PstI, Sag16I, SflI, YenI | |||||||
Ecl2zI | Enterobacter cloacae 2z |
5' CTGCAG 3' GACGTC
|
5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5'
|
AliAJI, Bsp63I, CfrA4I, PaePI, Pfl21I, PstI, SflI, Sst12I, YenI | |||||||
Eco24I | Escherichia coli RFL24 |
5' GRGCYC 3' CYCGRG
|
5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5'
|
||||||||
Eco31I | Escherichia coli RFL31 |
5' GGTCTC 3' CCAGAG
|
5' ---GGTCTCN NNNN--- 3' 3' ---CCAGAGNNNNN --- 5'
|
||||||||
Eco32I | Escherichia coli RFL32 |
5' GATATC 3' CTATAG
|
5' ---GAT ATC--- 3' 3' ---CTA TAG--- 5'
|
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Eco47I | Escherichia coli RFL47 |
5' GGWCC 3' CCWGG
|
5' ---G GWCC--- 3' 3' ---CCWG G--- 5'
|
Bme18I, BsrAI, Csp68KI, ErpI, FssI, HgiEI, Kzo49I, SmuEI | |||||||
Eco47III | Escherichia coli RFL47 |
5' AGCGCT 3' TCGCGA
|
5' ---AGC GCT--- 3' 3' ---TCG CGA--- 5'
|
AfeI, AitI, Aor51H, FunI | |||||||
Eco52I | Escherichia coli RFL52 |
5' CGGCCG 3' GCCGGC
|
5' ---C GGCCG--- 3' 3' ---GCCGG C--- 5'
|
AaaI, BseX3I, BstZI, EagI, EclXI, SenPT16I, XmaIII | |||||||
Eco56I | Escherichia coli RFL56 |
5' GCCGGC 3' CGGCCG
|
5' ---G CCGGC--- 3' 3' ---CGGCC G--- 5'
|
||||||||
Eco57I | Escherichia coli RFL57 |
5' CTGAAG 3' GACTTC
|
5' ---CTGAAGN12NNNN --- 3' 3' ---GACTTCN12NN NN--- 5'
|
AcuI, BspKT5I | |||||||
Eco64I | Escherichia coli RFL64 |
5' GGYRCC 3' CCRYGG
|
5' ---G GYRCC--- 3' 3' ---CCRYG G--- 5'
|
BanI, BbvBI, BspT107I, BshNI, HgiCI, HgiHI, MspB4I, PfaAI | |||||||
Eco72I | Escherichia coli RFL72 |
5' CACGTG 3' GTGCAC
|
5' ---CAC GTG--- 3' 3' ---GTG CAC--- 5'
|
AcvI, BcoAI, BbrPI, PmaCI, PmlI, PspCI | |||||||
Eco78I | Escherichia coli RFL78 |
5' GGCGCC 3' CCGCGG
|
5' ---GGC GCC--- 3' 3' ---CCG CGG--- 5'
|
||||||||
Eco81I | Escherichia coli RFL81 |
5' CCTNAGG 3' GGANTCC
|
5' ---CC TNAGG--- 3' 3' ---GGANT CC--- 5'
|
AxyI, Bse21I, BspR7I, Bsu36I, Eco81I, MstII, OxaNI, SshAI | |||||||
Eco88I | Escherichia coli RFL88 |
5' CYCGRG 3' GRGCYC
|
5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5'
|
AquI, BcoI, BsiHKCI, BstSI, Eco27kI, NspSAI, PlaAI, PunAI | |||||||
Eco91I | Escherichia coli RFL91 |
5' GGTNACC 3' CCANTGG
|
5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5'
|
Bse64I, BstEII, BstPI, BstT9I, BstT10I, EcaI, Eci125I, NspSAII | |||||||
Eco105I | Escherichia coli RFL105 |
5' TACGTA 3' ATGCAT
|
5' ---TAC GTA--- 3' 3' ---ATG CAT--- 5'
|
||||||||
Eco130I | Escherichia coli RFL130 |
5' CCWWGG 3' GGWWCC
|
5' ---C CWWGG--- 3' 3' ---GGWWC C--- 5'
|
||||||||
Eco147I | Escherichia coli RFL147 |
5' AGGCCT 3' TCCGGA
|
5' ---AGG CCT--- 3' 3' ---TCC GGA--- 5'
|
AatI, AspMI, GdiI, PceI, Pme55I, SarI, Sru30DI, SseBI, SteI, StuI | |||||||
Eco255I | Escherichia coli RFL255 |
5' AGTACT 3' TCATGA
|
5' ---AGT ACT--- 3' 3' ---TCA TGA--- 5'
|
|
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Eco1831I | Escherichia coli RFL1831 |
5' CCSGG 3' GGSCC
|
5' --- CCSGG--- 3' 3' ---GGSCC --- 5'
|
|
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EcoA4I | Escherichia coli A4 |
5' GGTCTC 3' CCAGAG
|
5' ---GGTCTCN NNNN--- 3' 3' ---CCAGAGNNNNN --- 5'
|
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EcoHI | Escherichia coli HI |
5' CCSGG 3' GGSCC
|
5' --- CCSGG--- 3' 3' ---GGSCC --- 5'
|
AsuC2I, BcnI, BpuMI, CauII, EcoHI, HgiS22I, Kpn49kII, NciI | |||||||
EcoHK31I | Escherichia coli HK31 |
5' YGGCCR 3' RCCGGY
|
5' ---Y GGCCR--- 3' 3' ---RCCGG Y--- 5'
|
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EcoICRI | Escherichia coli 2bT |
5' GAGCTC 3' CTCGAG
|
5' ---GAG CTC--- 3' 3' ---CTC GAG--- 5'
|
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Eco75KI | Escherichia coli BKM |
5' GRGCYC 3' CYCGRG
|
5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5'
|
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Eco57MI | Escherichia coli RFL57M |
5' CTGRAG 3' GACYTC
|
5' ---CTGRAGN12NNNN --- 3' 3' ---GACYTCN12NN NN--- 5'
|
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EcoNI | Escherichia coli |
5' CCTN5AGG 3' GGAN5TCC
|
5' ---CCTNN NNNAGG--- 3' 3' ---GGANNN NNTCC--- 5'
|
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EcoO44I | Escherichia coli O44 Hiromi |
5' GGTCTC 3' CCAGAG
|
5' ---GGTCTCN NNNN--- 3' 3' ---CCAGAGNNNNN --- 5'
|
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EcoO65I | Escherichia coli K11a |
5' GGTNACC 3' CCANTGG
|
5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5'
|
AcrII, Bse64I, BstEII, BstPI, BstT9I, EcaI, Eco91I, EcoO128I | |||||||
EcoO109I | 1WTD | Escherichia coli H709c |
5' RGGNCCY 3' YCCNGGR
|
5' ---RG GNCCY--- 3' 3' ---YCCNG GR--- 5'
|
|||||||
EcoO128I | Escherichia coli O128Ly3 |
5' GGTNACC 3' CCANTGG
|
5' ---G GTNACC--- 3' 3' ---CCANTG G--- 5'
|
AspAI, Bse64I, BstEII, BstT9I, EcaI, EcoO65I, NspSAII, PspEI | |||||||
EcoP15I | Escherichia coli P15 |
5' CAGCAG 3' GTCGTC
|
5' ---CAGCAGN25NN --- 3' 3' ---GTCGTCN25 NN--- 5'
|
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EcoR124II | 2y3t, 2w74 Search | Escherichia coli |
5' 5'-GAAN7RTCG-3'
|
5'
|
|||||||
EcoRI | 1QC9, Search EcoR1 structures | Escherichia coli RY13 |
5' GAATTC 3' CTTAAG
|
5' ---G AATTC--- 3' 3' ---CTTAA G--- 5'
|
|||||||
EcoRII | 1NA6 | Escherichia coli R245 |
5' CCWGG 3' GGWCC
|
5' --- CCWGG--- 3' 3' ---GGWCC --- 5'
|
ApaORI, BseBI, BspNI, BstNI, BstOI, Bst2UI, MvaI, SspAI, ZanI | ||||||
EcoRV | 1RVE Search EcoRV | Escherichia coli J62 pLG74 |
5' GATATC 3' CTATAG
|
5' ---GAT ATC--- 3' 3' ---CTA TAG--- 5'
|
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EcoT14I | Escherichia coli TB14 |
5' CCWWGG 3' GGWWCC
|
5' ---C CWWGG--- 3' 3' ---GGWWC C--- 5'
|
||||||||
EcoT22I | Escherichia coli TB22 |
5' ATGCAT 3' TACGTA
|
5' ---ATGCA T--- 3' 3' ---T ACGTA--- 5'
|
BfrBI, Csp68KIII, Mph1103I, NsiI, PinBI, Ppu10I, SepI, SspD5II | |||||||
EcoT38I | Escherichia coli TH38 |
5' GRGCYC 3' CYCGRG
|
5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5'
|
||||||||
EcoVIII | Escherichia coli E1585-68 |
5' AAGCTT 3' TTCGAA
|
5' ---A AGCTT--- 3' 3' ---TTCGA A--- 5'
|
||||||||
Eco13kI | Escherichia coli 13k |
5' CCNGG 3' GGNCC
|
5' --- CCNGG--- 3' 3' ---GGNCC --- 5'
|
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Eco21kI | Escherichia coli 21k |
5' CCNGG 3' GGNCC
|
5' --- CCNGG--- 3' 3' ---GGNCC --- 5'
|
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Eco27kI | Escherichia coli 27 |
5' CYCGRG 3' GRGCYC
|
5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5'
|
Ama87I, AvaI, Bse15I, BsoBI, BstSI, NspIII, OfoI, PunAI | |||||||
Eco29kI | Escherichia coli |
5' CCGCGG 3' GGCGCC
|
5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5'
|
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Eco53kI | Escherichia coli 53k |
5' GAGCTC 3' CTCGAG
|
5' ---GAG CTC--- 3' 3' ---CTC GAG--- 5'
|
||||||||
Eco137kI | Escherichia coli 137k |
5' CCNGG 3' GGNCC
|
5' --- CCNGG--- 3' 3' ---GGNCC --- 5'
|
||||||||
EgeI | Enterobacter gergoviae NA |
5' GGCGCC 3' CCGCGG
|
5' ---GGC GCC--- 3' 3' ---CCG CGG--- 5'
|
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EheI | Erwinia herbicola 9/5 |
5' GGCGCC 3' CCGCGG
|
5' ---GGC GCC--- 3' 3' ---CCG CGG--- 5'
|
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ErhI | Erwinia rhaponici B9 |
5' CCWWGG 3' GGWWCC
|
5' ---C CWWGG--- 3' 3' ---GGWWC C--- 5'
|
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ErhB9I | Erwinia rhaponici B9 |
5' CGATCG 3' GCTAGC
|
5' ---CGAT CG--- 3' 3' ---GC TAGC--- 5'
|
Afa16RI, Afa22MI, EagBI, NblI, Ple19I, Psu161I, RshI, XorII | |||||||
ErhB9II | Erwinia rhaponici B9 |
5' CCWWGG 3' GGWWCC
|
5' ---C CWWGG--- 3' 3' ---GGWWC C--- 5'
|
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ErpI | Erwinia rhaponici |
5' GGWCC 3' CCWGG
|
5' ---G GWCC--- 3' 3' ---CCWG G--- 5'
|
Bme18I, BthAI, Csp68KI, Eco47I, FssI, HgiEI, Kzo49I, SmuEI | |||||||
EsaBC3I | Environmental sample BC3 |
5' TCGA 3' AGCT
|
5' ---TC GA--- 3' 3' ---AG CT--- 5'
|
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EsaBC4I | Environmental sample BC4 |
5' GGCC 3' CCGG
|
5' ---GG CC--- 3' 3' ---CC GG--- 5'
|
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EspI | Eucapsis sp. |
5' GCTNAGC 3' CGANTCG
|
5' ---GC TNAGC--- 3' 3' ---CGANT CG--- 5'
|
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Esp3I | Erwinia sp. RFL3 |
5' CGTCTC 3' GCAGAG
|
5' ---CGTCTCN NNNN--- 3' 3' ---GCAGAGNNNNN --- 5'
|
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Esp4I | Erwinia sp. RFL4 |
5' CTTAAG 3' GAATTC
|
5' ---C TTAAG--- 3' 3' ---GAATT C--- 5'
|
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Esp1396I | Enterobacter sp. RFL1396 |
5' CCAN5TGG 3' GGTN5ACC
|
5' ---CCANNNN NTGG--- 3' 3' ---GGTN NNNNACC--- 5'
|
AccB7I, AcpII, Asp10HII, BasI, PflBI, PflMI, Van91I |
F
Enzyme | PDB code | Source | Recognition sequence | Cut | Isoschizomers | ||||
FalI | Flavobacterium aquatile Ob10 |
5' AAGN5CTT 3' TTCN5GAA
|
5' ---AAGN5CTTN7NNNNNN --- 3' 3' ---TTCN5GAAN7N NNNNN--- 5'
|
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FalII | Flavobacterium aquatile Ob10 |
5' CGCG 3' GCGC
|
5' ---CG CG--- 3' 3' ---GC GC--- 5'
|
BepI, BspFNI, Bsp123I, BspFNI, BstUI, BtkI, FalII, SelI, ThaI | |||||
FaqI | Flavobacterium aquatile RFL1 |
5' GGGAC 3' CCCTG
|
5' ---GGGACN8NN NNNN--- 3' 3' ---CCCTGN8NNNNNN --- 5'
|
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FatI | Flavobacterium aquatile NL3 |
5' CATG 3' GTAC
|
5' --- CATG--- 3' 3' ---GTAC --- 5'
|
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FauI | Flavobacterium aquatile |
5' CCCGC 3' GGGCG
|
5' ---CCCGCNNNN NN--- 3' 3' ---GGGCGNNNNNN --- 5'
|
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FauBII | Flavobacterium aureus B |
5' CGCG 3' GCGC
|
5' ---CG CG--- 3' 3' ---GC GC--- 5'
|
Bpu95I, BspFNI, Bsp123I, BspFNI, BstUI, BtkI, Csp68KVI, SelI, ThaI | |||||
FauNDI | Flavobacterium aquatile ND |
5' CATATG 3' GTATAC
|
5' ---CA TATG--- 3' 3' ---GTAT AC--- 5'
|
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FbaI | Flavobacterium balustinum |
5' TGATCA 3' ACTAGT
|
5' ---T GATCA--- 3' 3' ---ACTAG T--- 5'
|
|
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FblI | Flavobacterium balustinum |
5' GTMKAC 3' CAKMTG
|
5' ---GT MKAC--- 3' 3' ---CAKM TG--- 5'
|
AccI, XmiI | |||||
FbrI | Flavobacterium breve |
5' GCNGC 3' CGNCG
|
5' ---GC NGC--- 3' 3' ---CGN CG--- 5'
|
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FdiI | Fremyella diplosiphon |
5' GGWCC 3' CCWGG
|
5' ---G GWCC--- 3' 3' ---CCWG G--- 5'
|
Bme18I, BthAI, Csp68KI, ErpI, FssI, HgiEI, Kzo49I, SmuEI | |||||
FdiII | Fremyella diplosiphon |
5' TGCGCA 3' ACGCGT
|
5' ---TGC GCA--- 3' 3' ---ACG CGT--- 5'
|
Acc16I, AosI, AviII, FspI, MstI, NsbI, PamI, Pun14627I | |||||
FgoI | Fervidobacterium gondwanense AB39T |
5' CTAG 3' GATC
|
5' ---C TAG--- 3' 3' ---GAT C--- 5'
|
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FmuI | Flavobacterium multivorum |
5' GGNCC 3' CCNGG
|
5' ---GGNC C--- 3' 3' ---C CNGG--- 5'
|
FmuI AvcI, BavAII, Bce22I, Bsu54I, NspIV, Pde12I, UnbI | |||||
FnuAI | Fusobacterium nucleatum A |
5' GANTC 3' CTNAG
|
5' ---G ANTC--- 3' 3' ---CTNA G--- 5'
|
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FnuCI | Fusobacterium nucleatum C |
5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
Bme12I, Bsp67I, Bst19II, CcyI, FnuEI, MgoI, NphI, SauMI | |||||
FnuDI | Fusobacterium nucleatum D |
5' GGCC 3' CCGG
|
5' ---GG CC--- 3' 3' ---CC GG--- 5'
|
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FnuDII | Fusobacterium nucleatum D |
5' CGCG 3' GCGC
|
5' ---CG CG--- 3' 3' ---GC GC--- 5'
|
Bpu95I, Bsh1236I, Bsp50I, BstFNI, BstUI, Csp68KVI, FnuDII, ThaI | |||||
FnuDIII | Fusobacterium nucleatum D |
5' GCGC 3' CGCG
|
5' ---GCG C--- 3' 3' ---C GCG--- 5'
|
AspLEI, BspLAI, BstHHI, CfoI, HhaI, Hin6I, HinP1I, HspAI, SciNI | |||||
FnuEI | Fusobacterium nucleatum E |
5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
Bme12I, Bsp67I, Bst19II, CcyI, FnuCI, MgoI, NphI, Sau3AI | |||||
Fnu4HI | Fusobacterium nucleatum 4H |
5' GCNGC 3' CGNCG
|
5' ---GC NGC--- 3' 3' ---CGN CG--- 5'
|
||||||
FokI | 2FOK | Flavobacterium okeanokoites |
5' GGATG 3' CCTAC
|
5' ---GGATGN8N NNNN--- 3' 3' ---CCTACN8NNNNN --- 5'
|
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FriOI | Flavobacterium sp. O9 |
5' GRGCYC 3' CYCGRG
|
5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5'
|
||||||
FseI | Frankia sp. Eu11b |
5' GGCCGGCC 3' CCGGCCGG
|
5' ---GGCCGG CC--- 3' 3' ---CC GGCCGG--- 5'
|
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FsiI | Frankia sp. |
5' RAATTY 3' YTTAAR
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5' ---R AATTY--- 3' 3' ---YTTAA R--- 5'
|
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FspI | Fischerella sp. |
5' TGCGCA 3' ACGCGT
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5' ---TGC GCA--- 3' 3' ---ACG CGT--- 5'
|
Acc16I, AosI, AviII, FdiII, MstI, NsbI, PamI, Pun14627I | |||||
FspII | Fischerella sp. |
5' TTCGAA 3' AAGCTT
|
5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5'
|
Asp10HI, Bim19I, BsiCI, BstBI, Csp45I, LspI, PlaII, Ssp1I, SviI | |||||
Fsp1604I | Flavobacterium sp. I 16-04 |
5' CCWGG 3' GGWCC
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5' ---CC WGG--- 3' 3' ---GGW CC--- 5'
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ApaORI, BseBI, BshGI, BstNI, BstOI, Bst2UI, BthEI, EcoRII, SleI | |||||
FspAI | Flexibacter sp. TV-m21K |
5' RTGCGCAY 3' YACGCGTR
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5' ---RTGC GCAY--- 3' 3' ---YACG CGTR--- 5'
|
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FspBI | Flavobacterium sp. RFLI |
5' CTAG 3' GATC
|
5' ---C TAG--- 3' 3' ---GAT C--- 5'
|
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Fsp4HI | Flavobacterium sp. 4H |
5' GCNGC 3' CGNCG
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5' ---GC NGC--- 3' 3' ---CGN CG--- 5'
|
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FspMSI | Fischerella sp. |
5' GGWCC 3' CCWGG
|
5' ---G GWCC--- 3' 3' ---CCWG G--- 5'
|
Bme18I, BthAI, DsaIV, ErpI, HgiBI, HgiEI, Kzo49I, SmuEI | |||||
FssI | Fibrobacter succinogenes S85 |
5' GGWCC 3' CCWGG
|
5' ---G GWCC--- 3' 3' ---CCWG G--- 5'
|
Bme18I, BthAI, DsaIV, ErpI, FspMSI, HgiEI, Kzo49I, VpaK11AI | |||||
FunI | Fischerella uniformis |
5' AGCGCT 3' TCGCGA
|
5' ---AGC GCT--- 3' 3' ---TCG CGA--- 5'
|
AfeI, AitI, Aor51H, Eco47III | |||||
FunII | Fischerella uniformis |
5' GAATTC 3' CTTAAG
|
5' ---G AATTC--- 3' 3' ---CTTAA G--- 5'
|
Basic Concept | |
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Recognition sequence 4bp | |
Recognition sequence 5bp | |
Recognition sequence 6bp | |
Recognition sequence 8bp | |
Lists | |
* means cleavage produces blunt ends
|