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List of restriction enzyme cutting sites: G–K
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    List of restriction enzyme cutting sites: G–K

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    Legend of nucleobases
    Code Nucleotide represented
    A Adenine (A)
    C Cytosine (C)
    G Guanine (G)
    T Thymine (T)
    N A, C, G or T
    M A or C
    R A or G
    W A or T
    Y C or T
    S C or G
    K G or T
    H A, C or T
    B C, G or T
    V A, C or G
    D A, G or T

    This article contains a list of the most studied restriction enzymes whose names start with G to K inclusive. It contains approximately 90 enzymes.

    The following information is given:

    • Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature, and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
    • PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action.
    • Source: Organism that naturally produces the enzyme.
    • Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
    • Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site.
    • Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:
    as in this not listed enzyme:  EcoR70I 


    Whole list navigation

    Restriction enzymes

    G

    Enzyme PDB code Source Recognition sequence Cut Isoschizomers
    GalI Gluconobacter albidus 5' CCGCGG
    3' GGCGCC
    5' ---CCGC   GG--- 3'
    3' ---GG   CGCC--- 5'
    GceI Gluconobacter cerinus 5' CCGCGG
    3' GGCGCC
    5' ---CCGC   GG--- 3'
    3' ---GG   CGCC--- 5'
    GceGLI Gluconobacter cerinus 5' CCGCGG
    3' GGCGCC
    5' ---CCGC   GG--- 3'
    3' ---GG   CGCC--- 5'
    GdiI Gluconobacter dioxyacetonicus 5' AGGCCT
    3' TCCGGA
    5' ---AGG   CCT--- 3'
    3' ---TCC   GGA--- 5'
    AatI, AspMI, Eco147I, PceI, SarI, Sru30DI, SseBI, SteI, StuI
    GdiII Gluconobacter dioxyacetonicus 5' CGGCCR
    3' GCCGGY
    5' ---C   GGCCR--- 3'
    3' ---GCCGG   Y--- 5'
    GstI Geobacillus stearothermophilus 5' GGATCC
    3' CCTAGG
    5' ---G   GATCC--- 3'
    3' ---CCTAG   G--- 5'
    GsuI Gluconobacter suboxydans H-15T 5' CTGGAG
    3' GACCTC
    5' ---CTGGAGN12NNNN   --- 3'
    3' ---GACCTCN12NN   NN--- 5'

    H

    Enzyme PDB code Source Recognition sequence Cut Isoschizomers
    HacI Halococcus acetoinfaciens 5' GATC
    3' CTAG
    5' ---   GATC--- 3'
    3' ---CTAG   --- 5'
    Bme12I, Bsp67I, BstENII, CcyI, FnuEI, MgoI, NphI, Sau3AI
    HaeI Haemophilus aegyptius 5' WGGCCW
    3' WCCGGW
    5' ---WGG   CCW--- 3'
    3' ---WCC   GGW--- 5'
    HaeII Haemophilus aegypticus 5' RGCGCY
    3' YCGCGR
    5' ---RGCGC   Y--- 3'
    3' ---Y   CGCGR--- 5'
    AccB2I,  BfoI,  Bme142I,
    Bsp143II, BstH2I, LpnI
    HaeIII Haemophilus aegypticus 5' GGCC
    3' CCGG
    5' ---GG   CC--- 3'
    3' ---CC   GG--- 5'
    BsuRI
    HaeIV Haemophilus aegyptius 5' GAYN5RTC
    3' CTRN5YAG
    5' ---GAYN5RTCN8NNNNNN   --- 3'
    3' ---CTRN5YAGN8N   NNNNN--- 5'
    HalI Hafnia alvei B6 5' GAATTC
    3' CTTAAG
    5' ---G   AATTC--- 3'
    3' ---CTTAA   G--- 5'
    HalII Hafnia alvei B6 5' CTGCAG
    3' GACGTC
    5' ---CTGCA   G--- 3'
    3' ---G   ACGTC--- 5'
    AjoI, AliAJI, Bsp63I, CfrA4I, CfuII, CstI, PstI, SflI, Srl5DI, Sst12I
    HapII Haemophilus aphrophilus 5' CCGG
    3' GGCC
    5' ---C   CGG--- 3'
    3' ---GGC   C--- 5'
    HgaI Haemophilus gallinarum 5' GACGC
    3' CTGCG
    5' ---GACGCN4N   NNNNN--- 3'
    3' ---CTGCGN4NNNNNN   --- 5'
    HgiI Herpetosiphon giganteus 3303 5' GRCGYC
    3' CYGCRG
    5' ---GR   CGYC--- 3'
    3' ---CYGC   RG--- 5'
    AcyI, AsuIII, BbiII, BstACI, HgiGI, HgiHII, Hin1I, PamII
    HgiAI Herpetosiphon giganteus HP1023 5' GWGCWC
    3' CWCGWG
    5' ---GWGCW   C--- 3'
    3' ---C   WCGWG--- 5'
    Alw21I, AspHI, Bsh45I, BsiHKAI,
     Bsm6I, HpyF46II,  MspV281I
    HgiBI Herpetosiphon giganteus Hpg5 5' GGWCC
    3' CCWGG
    5' ---G   GWCC--- 3'
    3' ---CCWG   G--- 5'
    Bme18I, BthAI, DsaIV, ErpI, FspMSI, HgiEI, Psp03I, VpaK11AI
    HgiCI Herpetosiphon giganteus Hpg9 5' GGYRCC
    3' CCRYGG
    5' ---G   GYRCC--- 3'
    3' ---CCRYG   G--- 5'
    AccB1I, BanI, BspT107I, BshNI, Eco64I, HgiHI, MspB4I, PfaAI
    HgiCII Herpetosiphon giganteus Hpg9 5' GGWCC
    3' CCWGG
    5' ---G   GWCC--- 3'
    3' ---CCWG   G--- 5'
    Bme18I, BthAI, DsaIV, FspMSI, HgiHIII, Psp03I, VpaK11AI
    HgiCIII Herpetosiphon giganteus Hpg9 5' GTCGAC
    3' CAGCTG
    5' ---G   TCGAC--- 3'
    3' ---CAGCT   G--- 5'
    HgiDI Herpetosiphon giganteus Hpa2 5' GRCGYC
    3' CYGCRG
    5' ---GR   CGYC--- 3'
    3' ---CYGC   RG--- 5'
    AcyI, AsuIII, BbiII, BstACI, HgiGI, HgiI, Hin1I, PamII
    HgiDII Herpetosiphon giganteus Hpa2 5' GTCGAC
    3' CAGCTG
    5' ---G   TCGAC--- 3'
    3' ---CAGCT   G--- 5'
    HgiEI Herpetosiphon giganteus Hpg24 5' GGWCC
    3' CCWGG
    5' ---G   GWCC--- 3'
    3' ---CCWG   G--- 5'
    Bme216I, BthAI, DsaIV, ErpI, FspMSI, HgiHIII, VpaK11AI
    HgiGI Herpetosiphon giganteus Hpa1 5' GRCGYC
    3' CYGCRG
    5' ---GR   CGYC--- 3'
    3' ---CYGC   RG--- 5'
    AcyI, AosII, BbiII, BstACI, HgiI, HgiGI, Msp17I, PamII
    HgiHI Herpetosiphon giganteus HP1049 5' GGYRCC
    3' CCRYGG
    5' ---G   GYRCC--- 3'
    3' ---CCRYG   G--- 5'
    AccB1I, BanI, BspT107I, BshNI, Eco64I, HgiCI, MspB4I, PfaAI
    HgiHII Herpetosiphon giganteus HP1049 5' GRCGYC
    3' CYGCRG
    5' ---GR   CGYC--- 3'
    3' ---CYGC   RG--- 5'
    AosII, AsuIII, BsaHI, BstACI, HgiGI, HgiHII, Msp17I, PamII
    HgiHIII Herpetosiphon giganteus HP1049 5' GGWCC
    3' CCWGG
    5' ---G   GWCC--- 3'
    3' ---CCWG   G--- 5'
    Bme216I, DsaIV, ErpI, HgiBI, Psp03I, VpaK11AI, VpaK11BI
    HgiJI Herpetosiphon giganteus HFS101 5' GGWCC
    3' CCWGG
    5' ---G   GWCC--- 3'
    3' ---CCWG   G--- 5'
    Bme216I, CauI, DsaIV, FdiI, HgiBI, HgiHIII, Psp03I, VpaK11AI
    HgiJII Herpetosiphon giganteus HFS101 5' GRGCYC
    3' CYCGRG
    5' ---GRGCY   C--- 3'
    3' ---C   YCGRG--- 5'
    HgiS22I Herpetosiphon giganteus 5' CCSGG
    3' GGSCC
    5' ---CC   SGG--- 3'
    3' ---GGS   CC--- 5'
    AhaI, AseII, AsuC2I, BcnI, CauII, Eco1831I, EcoHI, Kpn49kII
    HhaI Haemophilus haemolyticus 5' GCGC
    3' CGCG
    5' ---GCG   C--- 3'
    3' ---C   GCG--- 5'
    AspLEI, BspLAI, BstHHI, CfoI, FnuDIII, Hin6I, HinP1I, SciNI
    HhaII Haemophilus haemolyticus 5' GANTC
    3' CTNAG
    5' ---G   ANTC--- 3'
    3' ---CTNA   G--- 5'
    Hin1I Haemophilus influenzae RFL1 5' GRCGYC
    3' CYGCRG
    5' ---GR   CGYC--- 3'
    3' ---CYGC   RG--- 5'
    AcyI, AhaII, AosII, AstWI, AsuIII, HgiI, HgiDI, HgiDI, Hsp92I
    Hin1II Haemophilus influenzae RFL1 5' CATG
    3' GTAC
    5' ---CATG   --- 3'
    3' ---   GTAC--- 5'
    Hin2I Haemophilus influenzae RFL2 5' CCGG
    3' GGCC
    5' ---C   CGG--- 3'
    3' ---GGC   C--- 5'
    Hin4I Haemophilus influenzae RFL4 5' GAYN5VTC
    3' CTRN5BAG
    5' ---GAYN5VTCN7NNNNNN   --- 3'
    3' ---CTRN5BAGN7N   NNNNN--- 5'
    Hin6I Haemophilus influenzae RFL6 5' GCGC
    3' CGCG
    5' ---G   CGC--- 3'
    3' ---CGC   G--- 5'
    AspLEI, BspLAI, BstHHI, CfoI, FnuDIII, HhaI, HsoI, HspAI, SciNI
    HinJCI Haemophilus influenzae JC9 5' GTYRAC
    3' CARYTG
    5' ---GTY   RAC--- 3'
    3' ---CAR   YTG--- 5'
    HinP1I 2FL3 Search PDBe Haemophilus influenzae P1 5' GCGC
    3' CGCG
    5' ---G   CGC--- 3'
    3' ---CGC   G--- 5'
    BspLAI, BstHHI, CfoI, FnuDIII, HhaI, Hin6I, HsoI, HspAI, SciNI
    HincII 2AUD Search PDBe Haemophilus influenzae Rc 5' GTYRAC
    3' CARYTG
    5' ---GTY   RAC--- 3'
    3' ---CAR   YTG--- 5'
    HindII Haemophilus influenzae Rd 5' GTYRAC
    3' CARYTG
    5' ---GTY   RAC--- 3'
    3' ---CAR   YTG--- 5'
    HindIII 2E52, Search PDBe Haemophilus influenzae Rd 5' AAGCTT
    3' TTCGAA
    5' ---A   AGCTT--- 3'
    3' ---TTCGA   A--- 5'
    HinfI Haemophilus influenzae Rf 5' GANTC
    3' CTNAG
    5' ---G   ANTC--- 3'
    3' ---CTNA   G--- 5'
    HjaI Hyphomonas jannaschiana 5' GATATC
    3' CTATAG
    5' ---GAT   ATC--- 3'
    3' ---CTA   TAG--- 5'
    HpaI Haemophilus parainfluenzae 5' GTTAAC
    3' CAATTG
    5' ---GTT   AAC--- 3'
    3' ---CAA   TTG--- 5'
    HpaII Haemophilus parainfluenzae 5' CCGG
    3' GGCC
    5' ---C   CGG--- 3'
    3' ---GGC   C--- 5'
    HphI Haemophilus parahaemolyticus 5' GGTGA
    3' CCACT
    5' ---GGTGAN6NN   --- 3'
    3' ---CCACTN6N   N--- 5'
    AsuHPI, SspD5I
    Hpy8I Helicobacter pylori A 8-5 5' GTNNAC
    3' CANNTG
    5' ---GTN   NAC--- 3'
    3' ---CAN   NTG--- 5'
    Hpy51I Helicobacter pylori 51 5' GTSAC
    3' CASTG
    5' ---   GTSAC--- 3'
    3' ---CASTG   --- 5'
    Hpy99I 3GOX Helicobacter pylori J99 5' CGWCG
    3' GCWGC
    5' ---CGWCG   --- 3'
    3' ---   GCWGC--- 5'
    Hpy178III Helicobacter pylori J178 5' TCNNGA
    3' AGNNCT
    5' ---TC   NNGA--- 3'
    3' ---AGNN   CT--- 5'
    Hpy188I Helicobacter pylori J188 5' TCNGA
    3' AGNCT
    5' ---TCN   GA--- 3'
    3' ---AG   NCT--- 5'
    Hpy188III Helicobacter pylori J188 5' TCNNGA
    3' AGNNCT
    5' ---TC   NNGA--- 3'
    3' ---AGNN   CT--- 5'
    HpyAV Helicobacter pylori 26695 5' CCTTC
    3' GGAAG
    5' ---CCTTCN4NN   --- 3'
    3' ---GGAAGN4N   N--- 5'
    HpyBI Helicobacter pylori Roberts 5' GTAC
    3' CATG
    5' ---GT   AC--- 3'
    3' ---CA   TG--- 5'
    AfaI, Csp6I, CviQI, CviRII, PabI, PlaAII, RsaI, RsaNI
    HpyBII Helicobacter pylori Roberts 5' GTNNAC
    3' CANNTG
    5' ---GTN   NAC--- 3'
    3' ---CAN   NTG--- 5'
    HpyCI Helicobacter pylori 5' GATATC
    3' CTATAG
    5' ---GAT   ATC--- 3'
    3' ---CTA   TAG--- 5'
    HpyC1I Helicobacter pylori 5' CCATC
    3' GGTAG
    5' ---CCATCNNNN   N--- 3'
    3' ---GGTAGNNNNN   --- 5'
    HpyCH4I Helicobacter pylori CH4 5' CATG
    3' GTAC
    5' ---CATG   --- 3'
    3' ---   GTAC--- 5'
    HpyCH4III Helicobacter pylori CH4 5' ACNGT
    3' TGNCA
    5' ---ACN   GT--- 3'
    3' ---TG   NCA--- 5'
    HpyCH4IV Helicobacter pylori CH4 5' ACGT
    3' TGCA
    5' ---A   CGT--- 3'
    3' ---TGC   A--- 5'
    HpyCH4V Helicobacter pylori CH4 5' TGCA
    3' ACGT
    5' ---TG   CA--- 3'
    3' ---AC   GT--- 5'
    HpyF10VI Helicobacter pylori RFL10 5' GCN7GC
    3' CGN7CG
    5' ---GCNNNNN   NNGC--- 3'
    3' ---CGNN   NNNNNCG--- 5'
    HpyF44III Helicobacter pylori RFL44 5' TGCA
    3' ACGT
    5' ---TG   CA--- 3'
    3' ---AC   GT--- 5'
    HsoI Haemophilus somnus 2336 5' GCGC
    3' CGCG
    5' ---G   CGC--- 3'
    3' ---CGC   G--- 5'
    AspLEI, BstHHI, CfoI, FnuDIII, Hin6I, HinP1I, HspAI, SciNI
    Hsp92I Haemophilus sp. 92 5' GRCGYC
    3' CYGCRG
    5' ---GR   CGYC--- 3'
    3' ---CYGC   RG--- 5'
    AcyI, AhaII, AosII, AstWI, HgiI, HgiDI, HgiHII, Hin1I, Hsp92I
    Hsp92II Haemophilus sp. 92 5' CATG
    3' GTAC
    5' ---CATG   --- 3'
    3' ---   GTAC--- 5'
    HspAI Haemophilus sp. A 5' GCGC
    3' CGCG
    5' ---G   CGC--- 3'
    3' ---CGC   G--- 5'
    AspLEI, BspLAI, BstHHI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, SciNI
    HsuI Haemophilus suis 5' AAGCTT
    3' TTCGAA
    5' ---A   AGCTT--- 3'
    3' ---TTCGA   A--- 5'

    I

    Enzyme PDB code Source Recognition sequence Cut Isoschizomers
    ItaI Ilyobcater tartaricus 5' GCNGC
    3' CGNCG
    5' ---GC   NGC--- 3'
    3' ---CGN   CG--- 5'

    K

    Enzyme PDB code Source Recognition sequence Cut Isoschizomers
    KasI Kluyvera ascorbata 5' GGCGCC
    3' CCGCGG
    5' ---G   GCGCC--- 3'
    3' ---CCGCG   G--- 5'
    Kaz48kI Klebsiella azeanae 5' RGGNCCY
    3' YCCNGGR
    5' ---RGGNC   CY--- 3'
    3' ---YC   CNGGR--- 5'
    KoxII Klebsiella oxytoca 5' GRGCYC
    3' CYCGRG
    5' ---GRGCY   C--- 3'
    3' ---C   YCGRG--- 5'
    KpnI Klebsiella pneumoniae OK8 5' GGTACC
    3' CCATGG
    5' ---GGTAC   C--- 3'
    3' ---C   CATGG--- 5'
    Acc65I, AhaB8I, Asp718I, SthI
    Kpn2I Klebsiella pneumoniae RFL2 5' TCCGGA
    3' AGGCCT
    5' ---T   CCGGA--- 3'
    3' ---AGGCC   T--- 5'
    Aor13HI, BbvAIII, BseAI, BsiMI, BspEI, BspMII, CauB3I, MroI
    Kpn378I Klebsiella pneumoniae 378 5' CCGCGG
    3' GGCGCC
    5' ---CCGC   GG--- 3'
    3' ---GG   CGCC--- 5'
    Kpn2kI Klebsiella pneumoniae 2k 5' CCNGG
    3' GGNCC
    5' ---   CCNGG--- 3'
    3' ---GGNCC   --- 5'
    Kpn49kI Klebsiella pneumoniae 49k 5' GAATTC
    3' CTTAAG
    5' ---G   AATTC--- 3'
    3' ---CTTAA   G--- 5'
    Kpn49kII Klebsiella pneumoniae 49k 5' CCSGG
    3' GGSCC
    5' ---   CCSGG--- 3'
    3' ---GGSCC   --- 5'
    AhaI, AsuC2I, BcnI, CauII, EcoHI, HgiS22I, Mgl14481I, NciI,
    KspI Kluyvera sp. 5' CCGCGG
    3' GGCGCC
    5' ---CCGC   GG--- 3'
    3' ---GG   CGCC--- 5'
    Ksp22I Kurthia sp. 22 5' TGATCA
    3' ACTAGT
    5' ---T   GATCA--- 3'
    3' ---ACTAG   T--- 5'
    AbaI, BclI, BsiQI,
    BspXII, BstT7I, FbaI,  ParI 
    Ksp632I Kluyvera sp. 632 5' CTCTTC
    3' GAGAAG
    5' ---CTCTTCN   NNN--- 3'
    3' ---GAGAAGNNNN   --- 5'
    KspAI Kurthia sp. N88 5' GTTAAC
    3' CAATTG
    5' ---GTT   AAC--- 3'
    3' ---CAA   TTG--- 5'
    Kzo9I Kurthia zopfii 9 5' GATC
    3' CTAG
    5' ---   GATC--- 3'
    3' ---CTAG   --- 5'
    Bce243I, Bsp105I, BspFI, BstMBI, CpfI, LlaAI, NdeII, Sth368I
    Kzo49I Kurthia zopfii 49 5' GGWCC
    3' CCWGG
    5' ---G   GWCC--- 3'
    3' ---CCWG   G--- 5'
    Bme216I, CauI, DsaIV, FdiI, HgiBI, HgiHIII, Psp03I, VpaK11AI

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