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List of restriction enzyme cutting sites: S
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Main articles: List of restriction enzyme cutting sites and Restriction enzyme
Legend of nucleobases | |
---|---|
Code | Nucleotide represented |
A | Adenine (A) |
C | Cytosine (C) |
G | Guanine (G) |
T | Thymine (T) |
N | A, C, G or T |
M | A or C |
R | A or G |
W | A or T |
Y | C or T |
S | C or G |
K | G or T |
H | A, C or T |
B | C, G or T |
V | A, C or G |
D | A, G or T |
This article contains a list of the most studied restriction enzymes whose names start with S. It contains approximately 130 enzymes. The following information is given:
- Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature, and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
- PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action.
- Source: Organism that naturally produces the enzyme.
- Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
- Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site.
- Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:
as in this not listed enzyme: EcoR70I
Whole list navigation
Restriction enzymes
S
Enzyme | PDB code | Source | Recognition sequence | Cut | Isoschizomers | ||||||
SacI | Streptomyces achromogenes |
5' GAGCTC 3' CTCGAG
|
5' ---GAGCT C--- 3' 3' ---C TCGAG--- 5'
|
||||||||
SacII | Streptomyces achromogenes |
5' CCGCGG 3' GGCGCC
|
5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5'
|
Cfr42I | |||||||
SacNI | Streptomyces achromogenes N-J-H |
5' GRGCYC 3' CYCGRG
|
5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5'
|
||||||||
SalI | Streptomyces albus |
5' GTCGAC 3' CAGCTG
|
5' ---G TCGAC--- 3' 3' ---CAGCT G--- 5'
|
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SalPI | Streptomyces albus |
5' CTGCAG 3' GACGTC
|
5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5'
|
ApiI, BspMAI, CstI, Ecl37kI, Ecl2zI, Psp23I, PstI, Srl5DI, YenI | |||||||
SanDI | Streptomyces sp. |
5' GGGWCCC 3' CCCWGGG
|
5' ---GG GWCCC--- 3' 3' ---CCCWG GG--- 5'
|
||||||||
SapI | Saccharopolyspora sp. |
5' GCTCTTC 3' CGAGAAG
|
5' ---GCTCTTCN NNN--- 3' 3' ---CGAGAAGNNNN --- 5'
|
||||||||
SarI | Streptomyces aureomonopodiales |
5' AGGCCT 3' TCCGGA
|
5' ---AGG CCT--- 3' 3' ---TCC GGA--- 5'
|
AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, Sru30DI, SseBI, SteI, StuI | |||||||
SatI | Staphylococcus arlettae RFL832 |
5' GCNGC 3' CGNCG
|
5' ---GC NGC--- 3' 3' ---CGN CG--- 5'
|
||||||||
SauI | Streptomyces aureofaciens IKA 18/4 |
5' CCTNAGG 3' GGANTCC
|
5' ---CC TNAGG--- 3' 3' ---GGANT CC--- 5'
|
AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI | |||||||
Sau96I | Staphylococcus aureus PS96 |
5' GGNCC 3' CCNGG
|
5' ---G GNCC--- 3' 3' ---CCNG G--- 5'
|
AsuI, Bal228I, Bce22I, BsiZI, BspF4I, Cfr13I, Nsp7121I, UnbI | |||||||
Sau3239I | Streptomyces aureofaciens |
5' CTCGAG 3' GAGCTC
|
5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5'
|
BspAAI, BstVI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaI | |||||||
Sau3AI | 2REU | Staphylococcus aureus 3A |
5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
Bce243I, Bsp143I, BspJI, BstMBI, CviAI, Kzo9I, NdeII, Sth368I | ||||||
SauBMKI | Streptomyces aureofaciens BM-K |
5' GCCGGC 3' CGGCCG
|
5' ---GCC GGC--- 3' 3' ---CGG CCG--- 5'
|
||||||||
SauHPI | Streptomyces aureofaciens R8/26 |
5' GCCGGC 3' CGGCCG
|
5' ---GCC GGC--- 3' 3' ---CGG CCG--- 5'
|
||||||||
SauLPI | Streptomyces aureofaciens B-96 |
5' GCCGGC 3' CGGCCG
|
5' ---GCC GGC--- 3' 3' ---CGG CCG--- 5'
|
||||||||
SauLPII | Streptomyces aureofaciens B-96 |
5' CTCGAG 3' GAGCTC
|
5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5'
|
BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaI | |||||||
SauMI | Staphylococcus aureus M |
5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
BscFI, BspAI, BstKTI, ChaI, HacI, MkrAI, RalF40I, SsiAI | |||||||
SauNI | Streptomyces aureofaciens NMU |
5' GCCGGC 3' CGGCCG
|
5' ---GCC GGC--- 3' 3' ---CGG CCG--- 5'
|
||||||||
SauSI | Streptomyces aureofaciens 16 |
5' GCCGGC 3' CGGCCG
|
5' ---GCC GGC--- 3' 3' ---CGG CCG--- 5'
|
||||||||
SbfI | Streptomyces sp. BF-61 |
5' CCTGCAGG 3' GGACGTCC
|
5' ---CCTGCA GG--- 3' 3' ---GG ACGTCC--- 5'
|
||||||||
Sbi68I | Streptomyces bikiniensis JAM68 |
5' CTCGAG 3' GAGCTC
|
5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5'
|
BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sol10179I, StrI, XpaI | |||||||
Sbo13I | Shigella boydii 13 |
5' TCGCGA 3' AGCGCT
|
5' ---TCG CGA--- 3' 3' ---AGC GCT--- 5'
|
||||||||
SbvI | Streptococcus bovis II/1 |
5' GGCC 3' CCGG
|
5' ---GG CC--- 3' 3' ---CC GG--- 5'
|
||||||||
ScaI | Streptomyces caespitosus |
5' AGTACT 3' TCATGA
|
5' ---AGT ACT--- 3' 3' ---TCA TGA--- 5'
|
|
|||||||
SceIII | Saccharomyces cerevisiae |
5' GCCGGC 3' CGGCCG
|
5' ---G CCGGC--- 3' 3' ---CGGCC G--- 5'
|
||||||||
SchI | Staphylococcus cohnii Lki 19-320 |
5' GAGTC 3' CTCAG
|
5' ---GAGTCN4N --- 3' 3' ---CTCAGN4N --- 5'
|
||||||||
SchZI | Streptomyces chusanensis ZS-2 |
5' CCGCGG 3' GGCGCC
|
5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5'
|
||||||||
SciI | Streptoverticillium cinnamoneum |
5' CTCGAG 3' GAGCTC
|
5' ---CTC GAG--- 3' 3' ---GAG CTC--- 5'
|
BspAAI, BstVI, MlaAI, PaeR7I, SauLPII, SlaI, Sol10179I, XhoI | |||||||
SciNI | Spiroplasma citri ASP2 |
5' GCGC 3' CGCG
|
5' ---G CGC--- 3' 3' ---CGC G--- 5'
|
BspLAI, BstHHI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI | |||||||
ScrFI | Streptococcus cremoris F |
5' CCNGG 3' GGNCC
|
5' ---CC NGG--- 3' 3' ---GGN CC--- 5'
|
||||||||
SdaI | 2IXS | Streptomyces diastaticus Ng7-324 |
5' CCTGCAGG 3' GGACGTCC
|
5' ---CCTGCA GG--- 3' 3' ---GG ACGTCC--- 5'
|
|||||||
SdiI | Streptomyces diastaticus |
5' GGCCN5GGCC 3' CCGGN5CCGG
|
5' ---GGCCNNNN NGGCC--- 3' 3' ---CCGGN NNNNCCGG--- 5'
|
||||||||
SduI | Streptococcus durans RFL3 |
5' GDGCHC 3' CHCGDG
|
5' ---GDGCH C--- 3' 3' ---C HCGDG--- 5'
|
AocII, BmyI, BsoCI, Bsp1286I, BspLS2I, MhlI, NspII | |||||||
SecI | Synechocystis sp. 6701 |
5' CCNNGG 3' GGNNCC
|
5' ---C CNNGG--- 3' 3' ---GGNNC C--- 5'
|
||||||||
SelI | Synechococcus elongatus |
5' CGCG 3' GCGC
|
5' --- CGCG--- 3' 3' ---GCGC --- 5'
|
AccII, Bsh1236I, Bsp123I, Bsp50I, BstFNI, BstUI, BtkI, Csp68KVI | |||||||
SenPT16I | Salmonella enteritidis PT16 |
5' CGGCCG 3' GCCGGC
|
5' ---C GGCCG--- 3' 3' ---GCCGG C--- 5'
|
AaaI, BseX3I, BstZI, EagI, EclXI, Eco52I, XmaIII | |||||||
SenPT14bI | Salmonella enteritidis PT14b |
5' CCGCGG 3' GGCGCC
|
5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5'
|
||||||||
SepI | Staphylococcus epidermidis |
5' ATGCAT 3' TACGTA
|
5' ---ATGCA T--- 3' 3' ---T ACGTA--- 5'
|
BfrBI, Csp68KIII, Mph1103I, NsiI, PinBI, Ppu10I, SspD5II, Zsp2I | |||||||
SexAI | Streptomyces exfoliatus |
5' ACCWGGT 3' TGGWCCA
|
5' ---A CCWGGT--- 3' 3' ---TGGWCC A--- 5'
|
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SexBI | Streptomyces exfoliatus |
5' CCGCGG 3' GGCGCC
|
5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5'
|
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SexCI | Streptomyces exfoliatus |
5' CCGCGG 3' GGCGCC
|
5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5'
|
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SfaI | Streptococcus faecalis zymogenes |
5' GGCC 3' CCGG
|
5' ---GG CC--- 3' 3' ---CC GG--- 5'
|
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SfaNI | Streptococcus faecalis ND547 |
5' GCATC 3' CGTAG
|
5' ---GCATCN4N NNNN--- 3' 3' ---CGTAGN4NNNNN --- 5'
|
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SfcI | Streptococcus faecium |
5' CTRYAG 3' GAYRTC
|
5' ---C TRYAG--- 3' 3' ---GAYRT C--- 5'
|
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SfeI | Streptococcus faecalis |
5' CTRYAG 3' GAYRTC
|
5' ---C TRYAG--- 3' 3' ---GAYRT C--- 5'
|
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SfiI | 2F03 | Streptomyces fimbriatus |
5' GGCCN5GGCC 3' CCGGN5CCGG
|
5' ---GGCCNNNN NGGCC--- 3' 3' ---CCGGN NNNNCCGG--- 5'
|
|||||||
SflI | Streptoverticillium flavopersicum |
5' CTGCAG 3' GACGTC
|
5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5'
|
ApiI, BspMAI, BsuBI, CfrA4I, Psp23I, PstI, Sag23I, Srl5DI, YenI | |||||||
SfoI | Serratia fonticola |
5' GGCGCC 3' CCGCGG
|
5' ---GGC GCC--- 3' 3' ---CCG CGG--- 5'
|
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Sfr274I | Streptomyces fradiae 274 |
5' CTCGAG 3' GAGCTC
|
5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5'
|
AbrI, BluI, BssHI, MavI, Sau3239I, Sol10179I, StrI, TliI | |||||||
Sfr303I | Streptomyces fradiae 303 |
5' CCGCGG 3' GGCGCC
|
5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5'
|
||||||||
SfuI | Streptomyces fulvissimus |
5' TTCGAA 3' AAGCTT
|
5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5'
|
AsuII, BimI, Bsp119I, CbiI, Csp68KII, PlaII, Ssp1I, SviI | |||||||
SgfI | Streptomyces griseoruber |
5' GCGATCGC 3' CGCTAGCG
|
5' ---GCGAT CGC--- 3' 3' ---CGC TAGCG--- 5'
|
|
|||||||
SgrAI | 3DPG 3N7B 3N78 3MGY 3MG6 | Streptomyces griseus |
5' CRCCGGYG 3' GYGGCCRC
|
5' ---CR CCGGYG--- 3' 3' ---GYGGCC RC--- 5'
|
|||||||
SgrBI | Streptomyces griseus |
5' CCGCGG 3' GGCGCC
|
5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5'
|
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SimI | Staphylococcus intermedius 6H |
5' GGGTC 3' CCCAG
|
5' ---GG GTC--- 3' 3' ---CCCAG --- 5'
|
||||||||
SinI | Salmonella infantis |
5' GGWCC 3' CCWGG
|
5' ---G GWCC--- 3' 3' ---CCWG G--- 5'
|
Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, Psp03I, VpaK11BI | |||||||
SlaI | Streptomyces lavendulae |
5' CTCGAG 3' GAGCTC
|
5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5'
|
AbrI, BluI, BssHI, MavI, Sau3239I, Sfr274I, TliI, XhoI | |||||||
SleI | Synechococcus leopoliensis |
5' CCWGG 3' GGWCC
|
5' --- CCWGG--- 3' 3' ---GGWCC --- 5'
|
AeuI, AglI, ApaORI, BspNI, Bst2I, EcoRII, SniI, SslI, SspAI, ZanI | |||||||
Slu1777I | Streptomyces lusitanus 1777 |
5' GCCGGC 3' CGGCCG
|
5' ---GCC GGC--- 3' 3' ---CGG CCG--- 5'
|
||||||||
SmaI | Serratia marcescens Sb |
5' CCCGGG 3' GGGCCC
|
5' ---CCC GGG--- 3' 3' ---GGG CCC--- 5'
|
AhyI, CfrJ4I, EaeAI, EclRI, Pac25I, PspAI, TspMI, XcyI, XmaI, XmaCI | |||||||
SmiI | Streptococcus milleri S |
5' ATTTAAAT 3' TAAATTTA
|
5' ---ATTT AAAT--- 3' 3' ---TAAA TTTA--- 5'
|
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SmiMI | Sphingobacterium mizutae M |
5' CAYN4RTG 3' GTRN4YAC
|
5' ---CAYNN NNRTG--- 3' 3' ---GTRNN NNYAC--- 5'
|
||||||||
SmlI | Stenotrophomonas maltophilia |
5' CTYRAG 3' GARYTC
|
5' ---C TYRAG--- 3' 3' ---GARYT C--- 5'
|
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SmuI | Sphingobacterium multivorum RFL21 |
5' CCCGC 3' GGGCG
|
5' ---CCCGCNNNN NN--- 3' 3' ---GGGCGNNNNNN --- 5'
|
||||||||
SmuEI | Streptococcus mutans E |
5' GGWCC 3' CCWGG
|
5' ---G GWCC--- 3' 3' ---CCWG G--- 5'
|
Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, Psp03I, VpaK11BI | |||||||
SnaBI | Sphaerotilus natans |
5' TACGTA 3' ATGCAT
|
5' ---TAC GTA--- 3' 3' ---ATG CAT--- 5'
|
||||||||
SniI | Streptomyces niveus |
5' CCWGG 3' GGWCC
|
5' ---CC WGG--- 3' 3' ---GGW CC--- 5'
|
ApaORI, BseBI, BshGI, BstNI, BstOI, Bst2UI, BthEI, MvaI, SspAI | |||||||
SnoI | Streptomyces novocastria |
5' GTGCAC 3' CACGTG
|
5' ---G TGCAC--- 3' 3' ---CACGT G--- 5'
|
Alw44I, ApaLI, VneI | |||||||
SolI | Streptoverticillium olivoverticillatum |
5' GGATCC 3' CCTAGG
|
5' ---G GATCC--- 3' 3' ---CCTAG G--- 5'
|
AccEBI, AliI, ApaCI, BamHI, Bce751I, CelI, OkrAI, SolI, SurI | |||||||
Sol10179I | Streptomyces olivochromogenes ST10179 |
5' CTCGAG 3' GAGCTC
|
5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5'
|
BspAAI, MavI, MlaAI, PaeR7I, Sbi68I, Sol10179I, StrI, XpaI | |||||||
SpaHI | Synechococus parietina |
5' GCATGC 3' CGTACG
|
5' ---GCATG C--- 3' 3' ---C GTACG--- 5'
|
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SpeI | Sphaerotilus natans |
5' ACTAGT 3' TGATCA
|
5' ---A CTAGT--- 3' 3' ---TGATC A--- 5'
|
AhII, AclNI, BcuI | |||||||
SphI | Streptomyces phaeochromogenes |
5' GCATGC 3' CGTACG
|
5' ---GCATG C--- 3' 3' ---C GTACG--- 5'
|
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SplI | Spirulina platensis |
5' CGTACG 3' GCATGC
|
5' ---C GTACG--- 3' 3' ---GCATG C--- 5'
|
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SpmI | Sphingobacterium multivorum RFL21 |
5' ATCGAT 3' TAGCTA
|
5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
|
BanIII, BbvAII, BdiI, BscI, Bsa29I, BspJI, ClaI, LcaI, PgaI, Ssp27144I | |||||||
SpoI | Salmonella potsdam |
5' TCGCGA 3' AGCGCT
|
5' ---TCG CGA--- 3' 3' ---AGC GCT--- 5'
|
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SpuI | Streptomyces pulveraceus |
5' CCGCGG 3' GGCGCC
|
5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5'
|
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SrfI | Streptomyces sp. |
5' GCCCGGGC 3' CGGGCCCG
|
5' ---GCCC GGGC--- 3' 3' ---CGGG CCCG--- 5'
|
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SrlI | Selenomonas ruminantium lactilytica 84 |
5' GCCGGC 3' CGGCCG
|
5' ---G CCGGC--- 3' 3' ---CGGCC G--- 5'
|
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Srl5DI | Selenomonas ruminantium 5 |
5' CTGCAG 3' GACGTC
|
5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5'
|
AliAJI, Asp713I, BspMAI, BsuBI, CfrA4I, Pfl21I, PstI, SflI, Srl5DI | |||||||
Srl32DII | Selenomonas ruminantium 32 |
5' GAATTC 3' CTTAAG
|
5' ---G AATTC--- 3' 3' ---CTTAA G--- 5'
|
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Srl55DI | Selenomonas ruminantium lactilytica 55 |
5' GAATTC 3' CTTAAG
|
5' ---G AATTC--- 3' 3' ---CTTAA G--- 5'
|
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Srl56DI | Selenomonas ruminantium 56 |
5' CTRYAG 3' GAYRTC
|
5' ---C TRYAG--- 3' 3' ---GAYRT C--- 5'
|
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SruI | Selenomonas ruminantium 18D |
5' TTTAAA 3' AAATTT
|
5' ---TTT AAA--- 3' 3' ---AAA TTT--- 5'
|
AhaIII, DraI, PauAII | |||||||
Sru4DI | Selenomonas ruminantium |
5' ATTAAT 3' TAATTA
|
5' ---AT TAAT--- 3' 3' ---TAAT TA--- 5'
|
AseI, AsnI, BpoAI, PshBI, VspI | |||||||
Sru30DI | Selenomonas ruminantium 30 |
5' AGGCCT 3' TCCGGA
|
5' ---AGG CCT--- 3' 3' ---TCC GGA--- 5'
|
AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, SarI, SseBI, SteI, StuI | |||||||
SsbI | Streptomyces scabies |
5' AAGCTT 3' TTCGAA
|
5' ---A AGCTT--- 3' 3' ---TTCGA A--- 5'
|
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SscL1I | Staphylococcus sp. L1 |
5' GANTC 3' CTNAG
|
5' ---G ANTC--- 3' 3' ---CTNA G--- 5'
|
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Sse9I | Sporosarcina sp. 9 |
5' AATT 3' TTAA
|
5' --- AATT--- 3' 3' ---TTAA --- 5'
|
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Sse232I | Streptomyces sp. RH232 |
5' CGCCGGCG 3' GCGGCCGC
|
5' ---CG CCGGCG--- 3' 3' ---GCGGCC GC--- 5'
|
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Sse1825I | Streptomyces sp. |
5' GGGWCCC 3' CCCWGGG
|
5' ---GG GWCCC--- 3' 3' ---CCCWG GG--- 5'
|
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Sse8387I | Streptomyces sp. 8387 |
5' CCTGCAGG 3' GGACGTCC
|
5' ---CCTGCA GG--- 3' 3' ---GG ACGTCC--- 5'
|
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Sse8647I | Streptomyces sp. |
5' AGGWCCT 3' TCCWGGA
|
5' ---AG GWCCT--- 3' 3' ---TCCWG GA--- 5'
|
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SseAI | Streptomyces sp. |
5' GGCGCC 3' CCGCGG
|
5' ---GG CGCC--- 3' 3' ---CCGC GG--- 5'
|
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SseBI | Streptomyces sp. |
5' AGGCCT 3' TCCGGA
|
5' ---AGG CCT--- 3' 3' ---TCC GGA--- 5'
|
AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, SarI, Sru30DI, SteI, StuI | |||||||
SshAI | Salmonella shikmonah |
5' CCTNAGG 3' GGANTCC
|
5' ---CC TNAGG--- 3' 3' ---GGANT CC--- 5'
|
AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI | |||||||
SsiI | Staphylococcus sciuri RFL1 |
5' CCGC 3' GGCG
|
5' ---C CGC--- 3' 3' ---GGC G--- 5'
|
AciI | |||||||
SsiAI | Staphylococcus sp. A |
5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
BscFI, BspAI, BstKTI, CpfI, HacI, MkrAI, RalF40I, SauMI | |||||||
SsiBI | Staphylococcus sp. B |
5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
BscFI, BspFI, BstKTI, CpfI, HacI, MkrAI, RalF40I, SauMI | |||||||
SslI | Streptococcus salivarius thermophilus |
5' CCWGG 3' GGWCC
|
5' ---CC WGG--- 3' 3' ---GGW CC--- 5'
|
ApaORI, BseBI, BshGI, BstNI, BstOI, Bst2UI, BthEI, EcoRII, MvaI | |||||||
SsoI | Shigella sonnei 47 |
5' GAATTC 3' CTTAAG
|
5' ---G AATTC--- 3' 3' ---CTTAA G--- 5'
|
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SsoII | Shigella sonnei 47 |
5' CCNGG 3' GGNCC
|
5' --- CCNGG--- 3' 3' ---GGNCC --- 5'
|
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SspI | Sphaerotilus sp. |
5' AATATT 3' TTATAA
|
5' ---AAT ATT--- 3' 3' ---TTA TAA--- 5'
|
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Ssp1I | Streptomyces sp. RFL1 |
5' TTCGAA 3' AAGCTT
|
5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5'
|
AsuII, BimI, Bsp119I, CbiI, MlaI, PlaII, SfuI, SspRFI, SviI | |||||||
Ssp4800I | Streptomyces sp. BMTU 4800 |
5' TGTACA 3' ACATGT
|
5' ---T GTACA--- 3' 3' ---ACATG T--- 5'
|
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Ssp5230I | Streptomyces sp. 5230 |
5' GACGTC 3' CTGCAG
|
5' ---GACGT C--- 3' 3' ---C TGCAG--- 5'
|
AatII, ZraI | |||||||
Ssp27144I | Synechococcus sp. |
5' ATCGAT 3' TAGCTA
|
5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
|
BanIII, BavCI, BbvAII, BscI, BspJI, BspOVII, BstNZ169I, ClaI, LcaI | |||||||
SspAI | Synechococcus sp. |
5' CCWGG 3' GGWCC
|
5' --- CCWGG--- 3' 3' ---GGWCC --- 5'
|
AeuI, AorI, Bse17I, BspNI, Bst2I, EcoRII, SniI, SslI, SspAI, ZanI | |||||||
SspBI | Streptomyces sp. |
5' TGTACA 3' ACATGT
|
5' ---T GTACA--- 3' 3' ---ACATG T--- 5'
|
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SspCI | Streptomyces sp. |
5' GCCGGC 3' CGGCCG
|
5' ---GCC GGC--- 3' 3' ---CGG CCG--- 5'
|
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SspD5I | Staphylococcus sp. D5 |
5' GGTGA 3' CCACT
|
5' ---GGTGAN7N --- 3' 3' ---CCACTN7N --- 5'
|
AsuHPI, HphI | |||||||
SspD5II | Staphylococcus sp. D5 |
5' ATGCAT 3' TACGTA
|
5' ---ATGCA T--- 3' 3' ---T ACGTA--- 5'
|
Csp68KIII, EcoT22I, Mph1103I, NsiI, PinBI, Ppu10I, SepI, Zsp2I | |||||||
SspRFI | Synechococcus sp. RF-1 |
5' TTCGAA 3' AAGCTT
|
5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5'
|
AsuII, BimI, Bsp119I, CbiI, MlaI, PpaAI, SfuI, Ssp1I, SviI | |||||||
SsrI | Staphylococcus saprophyticus B6 |
5' GTTAAC 3' CAATTG
|
5' ---GTT AAC--- 3' 3' ---CAA TTG--- 5'
|
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SstI | Streptomyces stanford |
5' GAGCTC 3' CTCGAG
|
5' ---GAGCT C--- 3' 3' ---C TCGAG--- 5'
|
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SstII | Streptomyces stanford |
5' CCGCGG 3' GGCGCC
|
5' ---CCGC GG--- 3' 3' ---GG CGCC--- 5'
|
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Sst12I | Streptomyces sp. ST-12 |
5' CTGCAG 3' GACGTC
|
5' ---CTGCA G--- 3' 3' ---G ACGTC--- 5'
|
Asp713I, BloHII, BspMAI, BsuBI, CfuII, Psp23I, PstI, SflI, Srl5DI | |||||||
SteI | Streptomyces tendae |
5' AGGCCT 3' TCCGGA
|
5' ---AGG CCT--- 3' 3' ---TCC GGA--- 5'
|
AatI, AspMI, Eco147I, GdiI, PceI, Pme55I, SarI, Sru30DI, SseBI, StuI | |||||||
SthI | Salmonella thompson YY356 |
5' GGTACC 3' CCATGG
|
5' ---G GTACC--- 3' 3' ---CCATG G--- 5'
|
Acc65I, AhaB8I, Asp718I, KpnI, | |||||||
Sth117I | Streptococcus thermophilus ST117 |
5' CCWGG 3' GGWCC
|
5' ---CC WGG--- 3' 3' ---GGW CC--- 5'
|
ApaORI, BseBI, BsiLI, BstNI, BstOI, Bst2UI, EcoRII, MvaI, SspAI | |||||||
Sth132I | Streptococcus thermophilus |
5' CCCG 3' GGGC
|
5' ---CCCGNNNN NNNN--- 3' 3' ---GGGCNNNNNNNN --- 5'
|
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Sth134I | Streptococcus thermophilus 134 |
5' CCGG 3' GGCC
|
5' ---C CGG--- 3' 3' ---GGC C--- 5'
|
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Sth368I | Streptococcus thermophilus CNRZ368 |
5' GATC 3' CTAG
|
5' --- GATC--- 3' 3' ---CTAG --- 5'
|
BscFI, BspFI, BstKTI, CpfI, HacI, MkrAI, RalF40I, SauMI | |||||||
StrI | Streptomyces thermodiastaticus |
5' CTCGAG 3' GAGCTC
|
5' ---C TCGAG--- 3' 3' ---GAGCT C--- 5'
|
AbrI, BluI, BssHI, MavI, Sau3239I, Sfr274I, TliI, XhoI | |||||||
StsI | Streptococcus sanguis 54 |
5' GGATG 3' CCTAC
|
5' ---GGATGN8NN NNNN--- 3' 3' ---CCTACN8NNNNNN --- 5'
|
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StuI | Streptomyces tubercidicus |
5' AGGCCT 3' TCCGGA
|
5' ---AGG CCT--- 3' 3' ---TCC GGA--- 5'
|
AatI, AspMI, Eco147I, GdiI, PceI, SarI, Sru30DI, SseBI, SteI | |||||||
StyI | Salmonella typhi |
5' CCWWGG 3' GGWWCC
|
5' ---C CWWGG--- 3' 3' ---GGWWC C--- 5'
|
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StyD4I | Salmonella typhi D4 |
5' CCNGG 3' GGNCC
|
5' --- CCNGG--- 3' 3' ---GGNCC --- 5'
|
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SuaI | Sulfolobus acidocaldarius |
5' GGCC 3' CCGG
|
5' ---GG CC--- 3' 3' ---CC GG--- 5'
|
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SuiI | Sulfolobus islandicus REN2HI |
5' GCWGC 3' CGWCG
|
5' ---G CWGC--- 3' 3' --- CGWC G--- 5'
|
AceI, ApeKI, Taq52I, TseI | |||||||
SunI | Synechococcus uniformis |
5' CGTACG 3' GCATGC
|
5' ---C GTACG--- 3' 3' ---GCATG C--- 5'
|
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SurI | Sporosarcina ureae 2 |
5' GGATCC 3' CCTAGG
|
5' ---G GATCC--- 3' 3' ---CCTAG G--- 5'
|
AccEBI, AliI, ApaCI, AsiI, BamHI, Bce751I, OkrAI, RspLKII, SolI | |||||||
SviI | Streptomyces violochromogenes |
5' TTCGAA 3' AAGCTT
|
5' ---TT CGAA--- 3' 3' ---AAGC TT--- 5'
|
AsuII, Bpu14I, Bsp119I, CbiI, MlaI, PpaAI, SfuI, Ssp1I | |||||||
SwaI | Staphylococcus warneri |
5' ATTTAAAT 3' TAAATTTA
|
5' ---ATTT AAAT--- 3' 3' ---TAAA TTTA--- 5'
|
Basic Concept | |
---|---|
Recognition sequence 4bp | |
Recognition sequence 5bp | |
Recognition sequence 6bp | |
Recognition sequence 8bp | |
Lists | |
* means cleavage produces blunt ends
|