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List of restriction enzyme cutting sites: A
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    List of restriction enzyme cutting sites: A

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    Legend of nucleobases
    Code Nucleotide represented
    A Adenine (A)
    C Cytosine (C)
    G Guanine (G)
    T Thymine (T)
    N A, C, G or T
    M A or C
    R A or G
    W A or T
    Y C or T
    S C or G
    K G or T
    H A, C or T
    B C, G or T
    V A, C or G
    D A, G or T

    This article contains a list of restriction enzymes whose names start with A and have a clearly defined cutting site.

    The following information is given for each enzyme:

    • Name of Restriction Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature, and bibliographical references. Note: When alphabetizing, enzymes are first ordered alphabetically by the acronyms (everything before the roman numeral); then enzymes of a given acronym are ordered alphabetically by the roman numeral, treating the numeral as a number and not a string of letters. This helps keep the entries ordered hierarchically while also alphabetic.(Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
    • PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action.
    • REBASE Number: Number used to identify restriction enzymes in the REBASE restriction enzyme database. This database includes important information about the enzyme such as Recognition sequence, source, and Isoschizomers, as well as other data, such as the commercial suppliers of the enzyme.
    • Source: Organism that naturally produces the enzyme.
    • Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
    • Cut: Displays the cut site and pattern and products of the cut. The recognition sequence and the cut site usually match, but sometimes the cut site can be dozens of nucleotides away from the recognition site.
    • Isoschizomers and neoschizomers: An isoschizomer is a restriction enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8–10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None as of [date]" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists, as in this not listed enzyme:  Abc123I 

    Whole list navigation

    Restriction enzymes

    A

    Enzyme PDB code REBASE Number Source Recognition sequence Cut Isoschizomers
    AaaI 1 Acetobacter aceti 5' CGGCCG
    3' GCCGGC
    5' ---C   GGCCG--- 3'
    3' ---GCCGG   C--- 5'
    BseX3I, BstZI, EagI, EclXI, Eco52I, SenPT16I, XmaIII
    AagI 4 Achromobacter agile 5' ATCGAT
    3' TAGCTA
    5' ---AT   CGAT--- 3'
    3' ---TAGC   TA--- 5'
    BanIII, BavCI, Bsa29I, BseCI, BspDI, Bsu15I, BsuTUI, ClaI
    AanI 15358 Arthrobacter aurescens RFL2 5' TTATAA
    3' AATATT
    5' ---TTA   TAA--- 3'
    3' ---AAT   ATT--- 5'
    PsiI, Csp1BORF7380P
    AarI 2892 Arthrobacter aurescens SS2-322 5' CACCTGC
    3' GTGGACG
    5' ---CACCTGCN3N   NNNN--- 3'
    3' ---GTGGACGN3NNNNN   --- 5'
    PaqCI
    AasI 5465 Arthrobacter aurescens RFL3 5' GACN6GTC
    3' CTGN6CAG
    5' ---GACNNNN   NNGTC--- 3'
    3' ---CTGNN   NNNNCAG--- 5'
    DrdI, DseDI
    AatI 6 Acetobacter aceti 5' AGGCCT
    3' TCCGGA
    5' ---AGG   CCT--- 3'
    3' ---TCC   GGA--- 5'
    AspMI, Eco147I,  Eco1524I,  PceI, Pme55I, SarI, Sru30DI, SteI
    AatII 7 Acetobacter aceti 5' GACGTC
    3' CTGCAG
    5' ---GACGT   C--- 3'
    3' ---C   TGCAG--- 5'
    Ssp5230I, ZraI
    AauI 3010 Arthrobacter aurescens 5' TGTACA
    3' ACATGT
    5' ---T   GTACA--- 3'
    3' ---ACATG   T--- 5'
     BsmRI,  Bsp1407I, BsrGI, BstAUI, Ssp4800I, SspBI
    AbaI 3029 Azospirillum brasilense UQ 1796 5' TGATCA
    3' ACTAGT
    5' ---T   GATCA--- 3'
    3' ---ACTAG   T--- 5'
    BclI, BsiQI, BspXII, BstT7I, FbaI, Ksp22I,  ParI 
    AbeI 3097 Azotobacter beijerinckii Slo 54-028 5' CCTCAGC
    3' GGAGTCG
    5' ---CC   TCAGC--- 3'
    3' ---GGAGT   CG--- 5'
    BbvCI
    AbrI 8 Azospirillum brasilense 5' CTCGAG
    3' GAGCTC
    5' ---C   TCGAG--- 3'
    3' ---GAGCT   C--- 5'
    BluI, BspAAI, BssHI, EscI, PaeR7I, SciI, Sfr274I, Sol10179I, StrI, TliI
    AbsI 14594 Arthrobacter sp. 7M06 5' CCTCGAGG
    3' GGAGCTCC
    5' ---CC   TCGAGG--- 3'
    3' ---GGAGCT   CC--- 5'
    None as of 4 Apr. 2019
    AccI 18 Acinetobacter calcoaceticus 5' GTMKAC
    3' CAKMTG
    5' ---GT   MKAC--- 3'
    3' ---CAKM   TG--- 5'
    FblI, XmiI
    AccII 19 Acinetobacter calcoaceticus 5' CGCG
    3' GCGC
    5' ---CG   CG--- 3'
    3' ---GC   GC--- 5'
    Bsh1236I, Bsp50I, BstFNI, BstUI, MvnI, SelI, BceBI, BepI, Bpu95I
    AccIII 20 Acinetobacter calcoaceticus 5' TCCGGA
    3' AGGCCT
    5' ---T   CCGGA--- 3'
    3' ---AGGCC   T--- 5'
    Aor13HI, BbvAIII, BlfI, BseAI, BsiMI, BspEI, CauB3I, Kpn2I, MroI, PtaI
    Acc16I 2638 Acinetobacter calcoaceticus 16 5' TGCGCA
    3' ACGCGT
    5' ---TGC   GCA--- 3'
    3' ---ACG   CGT--- 5'
    AosI, AviII, FdiII, FspI, MstI, NsbI, PamI, Pun14627I
    Acc36I 4162 Acinetobacter calcoaceticus 36 5' ACCTGC
    3' TGGACG
    5' ---ACCTGCN3N   NNNN--- 3'
    3' ---TGGACGN3NNNNN   --- 5'
    BfuAI, BspMI, BveI
    Acc65I 14 Acinetobacter calcoaceticus 65 5' GGTACC
    3' CCATGG
    5' ---G   GTACC--- 3'
    3' ---CCATG   G--- 5'
    AhaB8I, Asp718I, KpnI, SthI
    Acc113I 2665 Acinetobacter calcoaceticus 113 5' AGTACT
    3' TCATGA
    5' ---AGT   ACT--- 3'
    3' ---TCA   TGA--- 5'
    AssI,  BmcAI, Bpa34I,  DpaI, Eco255I, RflFII, ScaI, ZrmI
    AccB1I 15 Acinetobacter calcoaceticus B1 5' GGYRCC
    3' CCRYGG
    5' ---G   GYRCC--- 3'
    3' ---CCRYG   G--- 5'
    BanI, BbvBI, BshNI, BspT107I, Eco64I, HgiCI, HgiHI, MspB4I
    AccB2I 2666 Acinetobacter calcoaceticus B2 5' RGCGCY
    3' YCGCGR
    5' ---RGCGC   Y--- 3'
    3' ---Y   CGCGR--- 5'
     BfoI,  Bme142I, Bsp143II, BstH2I, HaeII, LpnI
    AccB7I 16 Acinetobacter calcoaceticus B7 5' CCAN5TGG
    3' GGTN5ACC
    5' ---CCANNNN   NTGG--- 3'
    3' ---GGTN   NNNNACC--- 5'
    AcpII, Asp10HII, BasI, Esp1396I, PflBI, PflMI, Van91I
    AccBSI 2733 Acinetobacter calcoaceticus BS 5' CCGCTC
    3' GGCGAG
    5' ---CCG   CTC--- 3'
    3' ---GGC   GAG--- 5'
    BsrBI, BstD102I, Bst31NI, MbiI
    AccEBI 17 Acinetobacter calcoaceticus EBF 65/65 5' GGATCC
    3' CCTAGG
    5' ---G   GATCC--- 3'
    3' ---CCTAG   G--- 5'
    AsiI, BamHI, Bce751I, Bsp98I, BspAAIII, CelI, OkrAI, Uba4009I
    AceI 2571 Anabaena cedrorum 5' GCWGC
    3' CGWCG
    5' ---G   CWGC--- 3'
    3' ---CGWC   G--- 5'
    ApeKI, SuiI, Taq52I, TseI
    AceII 2570 Anabaena cedrorum 5' GCTAGC
    3' CGATCG
    5' ---GCTAG   C--- 3'
    3' ---C   GATCG--- 5'
    AsuNHI, BmtI,  BspOI, , LlaG2I, NheI, PstNHI
    AceIII 2569 Anabaena cedrorum 5' CAGCTC
    3' GTCGAG
    5' ---CAGCTCN5N   NNNN--- 3'
    3' ---GTCGAGN5NNNNN   --- 5'
    — None as of May 2010 —
    AciI 21 Arthrobacter citreus 5' CCGC
    3' GGCG
    5' ---C   CGC--- 3'
    3' ---GGC   G--- 5'
    SsiI
    AclI 2181 Acinetobacter calcoaceticus M4 5' AACGTT
    3' TTGCAA
    5' ---AA   CGTT--- 3'
    3' ---TTGC   AA--- 5'
    Psp1406I
    AclNI 2667 Acinetobacter calcoaceticus N20 5' ACTAGT
    3' TGATCA
    5' ---A   CTAGT--- 3'
    3' ---TGATC   A--- 5'
    AhlI, BcuI, SpeI
    AclWI 2668 Acinetobacter calcoaceticus W2131 5' GGATC
    3' CCTAG
    5' ---GGATCN3N   N--- 3'
    3' ---CCTAGN3NN   --- 5'
    AlwI, BinI,  BpuFI, BsrWI,  BspPI, BstH9I,  Bst31TI,  EacI,  Ral8I 
    AcoI 11822 Acinetobacter calcoaceticus 5' YGGCCR
    3' RCCGGY
    5' ---Y   GGCCR--- 3'
    3' ---RCCGG   Y--- 5'
    EaeI, Bfi89I, CfrI, EcoHK31I
    AcpI 22 Acidiphilium cryptum 25H 5' TTCGAA
    3' AAGCTT
    5' ---TT   CGAA--- 3'
    3' ---AAGC   TT--- 5'
    Asp10HI, Bpu14I, Bsp119I, BstBI, Csp45I, FspII, MlaI, NspV, SspRFI
    AcpII 23 Acidiphilium cryptum 25H 5' CCAN5TGG
    3' GGTN5ACC
    5' ---CCANNNN   NTGG--- 3'
    3' ---GGTN   NNNNACC--- 5'
    AccB7I, Asp10HII, BasI, Esp1396I, PflBI, PflMI, Van91I
    AcrII 25 Anabaenopsis circularis 5' GGTNACC
    3' CCANTGG
    5' ---G   GTNACC--- 3'
    3' ---CCANTG   G--- 5'
    AspAI, Bse64I, BseT9I, BstEII, BstPI, Eco91I, EcoO65I, PspEI
    AcsI 2184 Arthrobacter citreus 310 5' RAATTY
    3' YTTAAR
    5' ---R   AATTY--- 3'
    3' ---YTTAA   R--- 5'
    ApoI,  CfaI,  FsiI, XapI
    AcuI 5534 Acinetobacter calcoaceticus 5' CTGAAG
    3' GACTTC
    5' ---CTGAAGN13NNN   --- 3'
    3' ---GACTTCN13N   NN--- 5'
    BspKT5I, Eco57I
    AcvI 5359 Aeromonas caviae 5' CACGTG
    3' GTGCAC
    5' ---CAC   GTG--- 3'
    3' ---GTG   CAC--- 5'
    BbrPI, BcoAI, Eco72I, PmaCI, PmlI, PspCI
    AcyI 31 Anabaena cylindrica 5' GRCGYC
    3' CYGCRG
    5' ---GR   CGYC--- 3'
    3' ---CYGC   RG--- 5'
    AhaII, AosII, BbiII, BsaHI, BstACI, Hin1I, HgiI, Hsp92I, Msp17I
    AdeI 2991 Alcaligenes denitrificans Ss 3-028 5' CACNNNGTG
    3' GTGNNNCAC
    5' ---CACNNN   GTG--- 3'
    3' ---GTG   NNNCAC--- 5'
    BstIZ316I, DraIII
    AeuI 33 Achromobacter eurydice 5' CCWGG
    3' GGWCC
    5' ---CC   WGG--- 3'
    3' ---GGW   CC--- 5'
    AglI, AjnI, BseBI, BstNI, BstOI, Bst2UI, EcoRII, SleI, SspAI
    AfaI 37 Acidiphilium facilis 28H 5' GTAC
    3' CATG
    5' ---GT   AC--- 3'
    3' ---CA   TG--- 5'
    Csp6I, CviQI, CviRII, HpyBI, PabI, PlaAII, RsaI, RsaNI
    Afa16RI 34 Acidiphilium sp. 16R 5' CGATCG
    3' GCTAGC
    5' ---CGAT   CG--- 3'
    3' ---GC   TAGC--- 5'
    Afa22MI, BspCI, EagBI, MvrI, NblI, Ple19I, PvuI, Psu161I, RshI, XorII
    Afa22MI 35 Acidocella facilis 5' CGATCG
    3' GCTAGC
    5' ---CGAT   CG--- 3'
    3' ---GC   TAGC--- 5'
    Afa16RI, BspCI, EagBI, ErhB9I, NblI, Ple19I, Psu161I, PvuI, RshI
    AfeI 2669 Alcaligenes faecalis T2774 5' AGCGCT
    3' TCGCGA
    5' ---AGC   GCT--- 3'
    3' ---TCG   CGA--- 5'
    AitI, Aor51H, Eco47III, FunI
    AfiI 10489 Anoxybacillus flavithermus 5' CCN7GG
    3' GGN7CC
    5' ---CCNNNNN   NNGG--- 3'
    3' ---GGNN   NNNNNCC--- 5'
    Bsc4I, BseLI, BslI, BflI, Bsc107I, BsiYI, Bst22I
    AflI Anabaena flos-aquae 5' GGWCC
    3' CCWGG
    5' ---G   GWCC--- 3'
    3' ---CCWG   G--- 5'
    Asp745I, BamNI, BcuAI, CauI, Csp68KI, DsaIV, Eco47I, SinI
    AflII 39 Anabaena flos-aquae 5' CTTAAG
    3' GAATTC
    5' ---C   TTAAG--- 3'
    3' ---GAATT   C--- 5'
    BfrI, BspTI, Bst98I, BstAFI, BstPZ740I, Esp4I, MspCI, Vha464I
    AflIII 40 Anabaena flos-aquae 5' ACRYGT
    3' TGYRCA
    5' ---A   CRYGT--- 3'
    3' ---TGYRC   A--- 5'
     Asp90I 
    AgeI 42 Agrobacterium gelatinovorum 5' ACCGGT
    3' TGGCCA
    5' ---A   CCGGT--- 3'
    3' ---TGGCC   A--- 5'
    AsiAI, AsiGI, BshTI, CsiAI, CspAI, PinAI
    AglI 6652 Arthrobacter globiformis 5' CCWGG
    3' GGWCC
    5' ---CC   WGG--- 3'
    3' ---GGW   CC--- 5'
    AeuI, Bse16I, Bse17I, Bse24I, BshGI, EcoRII, SleI, SniI, SslI
    AgsI Agrococcus sp. 25 5' TTSAA
    3' AASTT
    5' ---TTS   AA--- 3'
    5' ---AA   STT--- 3'
    None as of 4 May 2019
    AhaI 45 Alteromonas haloplanktis B8 5' CCSGG
    3' GGSCC
    5' ---CC   SGG--- 3'
    3' ---GGS   CC--- 5'
    AseII, BcnI,  BpuMI,  CauII, Eco1831I, EcoHI, HgiS22I, NciI
    AhaII Aphanothece halophytica 5' GRCGYC
    3' CYGCRG
    5' ---GR   CGYC--- 3'
    3' ---CYGC   RG--- 5'
    AcyI, AstWI, AsuIII, BsaHI, BstACI, Hin1I, HgiI, HgiDI, HgiHII, PamII
    AhaIII Aphanothece halophytica 5' TTTAAA
    3' AAATTT
    5' ---TTT   AAA--- 3'
    3' ---AAA   TTT--- 5'
    DraI, PauAII, SruI
    AhaB8I Aphanothece halophytica B8 5' GGTACC
    3' CCATGG
    5' ---G   GTACC--- 3'
    3' ---CCATG   G--- 5'
    Acc65I, Asp718I, KpnI, SthI
    AhdI Aeromonas hydrophila 5' GACN5GTC
    3' CTGN5CAG
    5' ---GACNNN   NNGTC--- 3'
    3' ---CTGNN   NNNCAG--- 5'
    AspEI,  BmeRI,  BspOVI, DriI, Eam1105I, EclHKI, NruGI
    AhlI 4838 Alteromonas haloplanktis SP 5' ACTAGT
    3' TGATCA
    5' ---A   CTAGT--- 3'
    3' ---TGATC   A--- 5'
    AclNI, BcuI, SpeI
    AhyI 49 Aeromonas hydrophila 5' CCCGGG
    3' GGGCCC
    5' ---C   CCGGG--- 3'
    3' ---GGGCC   C--- 5'
    Cfr9I, CfrJ4I, EaeAI, EclRI, PaeBI, SmaI, TspMI, XmaI, XmaCI
    AitI 54 Aquaspirillum itersonii 5' AGCGCT
    3' TCGCGA
    5' ---AGC   GCT--- 3'
    3' ---TCG   CGA--- 5'
    AfeI, Aor51H, Eco47III, FunI
    AjiI 10919 Acinetobacter johnsonii RFL47 5' CACGTC
    3' GTGCAG
    5' ---CAC   GTC--- 3'
    3' ---GTG   CAG--- 5'
    BmgBI, BtrI
    AjnI 7185 Acinetobacter johnsonii R2 5' CCWGG
    3' GGWCC
    5' ---   CCWGG--- 3'
    3' ---GGWCC   --- 5'
    AorI, ApaORI, ApyI, EcoRII, MvaI, Psp6I, PspGI, SleI, SspAI
    AjoI 2604 Acinetobacter johnsonii 35 5' CTGCAG
    3' GACGTC
    5' ---CTGCA   G--- 3'
    3' ---G   ACGTC--- 5'
    ApiI, BloHII, BspMAI, CfrA4I, CfuII, PstI, SalPI, SflI, Srl5DI
    AjuI 7740 Acinetobacter junii RFL46 5' GAAN7TTGG
    3'CTTN7AACC
    5' ---NNNNN   NN6GAAN7TTGGN5NNNNNN   --- 3'
    3' ---   NNNNNNN6CTTN7AACCN5N   NNNNN--- 5'
    None as of 28 Oct. 2020
    AleI 5791 Aureobacterium liquefaciens 5' CACN4GTG
    3' GTGN4CAC
    5' ---CACNN   NNGTG--- 3'
    3' ---GTGNN   NNCAC--- 5'
    OliI
    AlfI 6110 Acinetobacter lwoffii BH 32 5' GCAN6TGC
    3' CGTN6ACG
    5' ---GCAN6TGCN9NNN   --- 3'
    3' ---CGTN6ACGN9N   NN--- 5'
    None as of May 2010
    AliI Acetobacter liquefaciens 5' GGATCC
    3' CCTAGG
    5' ---G   GATCC--- 3'
    3' ---CCTAG   G--- 5'
    AccEBI, BamHI, Bce751I, Bsp98I, BspAAIII, Nsp29132II, Pfl8I
    AliAJI 59 Acetobacter liquefaciens AJ 2881 5' CTGCAG
    3' GACGTC
    5' ---CTGCA   G--- 3'
    3' ---G   ACGTC--- 5'
    ApiI, Asp713I, BspMAI, CflI, CstI, MhaAI, PstI, SalPI, Sst12I, YenI
    AloI 2812 Acinetobacter lwoffii Ks 4-8 5' GAACN6TCC
    3' CTTGN6AGG
    5' ---GAACN6TCCN6NNNNNN   --- 3'
    3' ---CTTGN6AGGN6N   NNNNN--- 5'
    None as of May 2010
    AluI 61 Arthrobacter luteus 5' AGCT
    3' TCGA
    5' ---AG   CT--- 3'
    3' ---TC   GA--- 5'
    AluBI, MltI
    AluBI 16189 Arthrobacter luteus B 5' AGCT
    3' TCGA
    5' ---AG   CT--- 3'
    3' ---TC   GA--- 5'
    AluI, MltI
    AlwI 65 Acinetobacter lwoffii 5' GGATC
    3' CCTAG
    5' ---GGATCN3N   N--- 3'
    3' ---CCTAGN3NN   --- 5'
    AclWI, BinI, BspPI,  BsrWI,  BstH9I,  BthII, Bth617I,  EacI
    Alw21I Acinetobacter lwoffii RFL21 5' GWGCWC
    3' CWCGWG
    5' ---GWGCW   C--- 3'
    3' ---C   WCGWG--- 5'
    AspHI, Bbv12I, Bsh45I, BsiHKAI, Bsm6I,  HgiAI, MspV281I
    Alw26I 63 Acinetobacter lwoffii RFL26 5' GTCTC
    3' CAGAG
    5' ---GTCTCN   NNNN--- 3'
    3' ---CAGAGNNNNN   --- 5'
     BcoDI, BscQII,   BsmAI, BsoMAI,  BstMAI 
    Alw44I Acinetobacter lwoffii RFL44 5' GTGCAC
    3' CACGTG
    5' ---G   TGCAC--- 3'
    3' ---CACGT   G--- 5'
    ApaLI, SnoI, VneI
    AlwNI Acinetobacter lwoffii N 5' CAGNNNCTG
    3' GTCNNNGAC
    5' ---CAGNNN   CTG--- 3'
    3' ---GTC   NNNGAC--- 5'
    CaiI
    AlwXI 67 Acinetobacter lwoffii X 5' GCAGC
    3' CGTCG
    5' ---GCAGCN7N   NNNN--- 3'
    3' ---CGTCGN7NNNNN   --- 5'
    BbvI, BseKI, BseXI, Bsp423I, Bst12I, Bst71I, BstV1I
    Ama87I Alteromonas macleodii 87 5' CYCGRG
    3' GRGCYC
    5' ---C   YCGRG--- 3'
    3' ---GRGCY   C--- 5'
    AquI, AvaI,  BmeT110I,  BsiHKCI, BsoBI, Eco88I, Nli3877I, NspSAI
    AmaCSI 26714 Arthrospira maxima CS-328 5' GCTCCA
    3' CGAGGT
    5' ---GCTCCAN8NNN   --- 3'
    3' ---CGAGGTN8N   NN--- 5'
    None as of 4 May 2018
    AocI 74 Anabaena sp. 5' CCTNAGG
    3' GGANTCC
    5' ---CC   TNAGG--- 3'
    3' ---GGANT   CC--- 5'
    AyI, Bse21I, Bsu36I, Eco81I, Lmu60I, OaNI, MstII, SauI, SshAI
    AocII Anabaena sp. 5' GDGCHC
    3' CHCGDG
    5' ---GDGCH   C--- 3'
    3' ---C   HCGDG--- 5'
    BmyI, BsoCI, Bsp1286I, BspLS2I, MhlI, NspII, SduI
    AorI 77 Acetobacter aceti orleanensis 5' CCWGG
    3' GGWCC
    5' ---CC   WGG--- 3'
    3' ---GGW   CC--- 5'
    BseBI, BstNI, BstOI, Bst2UI, EcoRII, MvaI, Sth117I, Tai
    Aor13HI Acidiphilium organovorum 13H 5' TCCGGA
    3' AGGCCT
    5' ---T   CCGGA--- 3'
    3' ---AGGCC   T--- 5'
    AccIII, BbvAIII, BlfI, BseAI, BsiMI, BspEI, CauB3I, Kpn2I, MroI, PtaI
    Aor51HI 76 Acidiphilium organovorum 51H 5' AGCGCT
    3' TCGCGA
    5' ---AGC   GCT--- 3'
    3' ---TCG   CGA--- 5'
    AfeI, AitI, Eco47III, FunI
    AosI Anabaena oscillarioides 5' TGCGCA
    3' ACGCGT
    5' ---TGC   GCA--- 3'
    3' ---ACG   CGT--- 5'
    Acc16I, AviII, FdiII, FspI, MstI, NsbI, PamI, Pun14627I
    AosII Anabaena oscillarioides 5' GRCGYC
    3' CYGCRG
    5' ---GR   CGYC--- 3'
    3' ---CYGC   RG--- 5'
    AcyI, AstWI, BbiII, BsaHI, BstACI, Hin1I, HgiI, HgiGI, Msp17I, PamII
    ApaI Acetobacter pasteurianus pasteurianus 5' GGGCCC
    3' CCCGGG
    5' ---GGGCC   C--- 3'
    3' ---C   CCGGG--- 5'
    Bsp120I, PpeI, PspOMI
    ApaBI Acetobacter pasteurianus B 5' GCAN5TGC
    3' CGTN5ACG
    5' ---GCANNNNN   TGC--- 3'
    3' ---CGT   NNNNNACG--- 5'
    BstAPI
    ApaCI Acetobacter pasteurianus C 5' GGATCC
    3' CCTAGG
    5' ---G   GATCC--- 3'
    3' ---CCTAG   G--- 5'
    AliI, BamHI, BnaI, Bsp4009I, BstI, NspSAIV, Pfl8I, SolI, SurI
    ApaLI Acetobacter pasteurianus 5' GTGCAC
    3' CACGTG
    5' ---G   TGCAC--- 3'
    3' ---CACGT   G--- 5'
    Alw44I, SnoI, VneI
    ApaORI 86 Acetobacter pasteurianus 5' CCWGG
    3' GGWCC
    5' ---CC   WGG--- 3'
    3' ---GGW   CC--- 5'
    AjnI, BstNI, BstOI, Bst1I, Bst2I, Fsp1604I, MvaI, Psp6I, PspGI
    ApeKI Aeropyrum pernix K1 5' GCWGC
    3' CGWCG
    5' ---G   CWGC--- 3'
    3' ---CGWC   G--- 5'
    AceI, SuiI, Taq52I, TseI
    ApiI 2315 Arthrobacter picolinophilus 5' CTGCAG
    3' GACGTC
    5' ---CTGCA   G--- 3'
    3' ---G   ACGTC--- 5'
    Asp713I, Bsp63I, CfrA4I, PaePI, Pfl21I, Psp23I, PstI, SflI, Sst12I
    ApoI Arthrobacter protophormiae 5' RAATTY
    3' YTTAAR
    5' ---R   AATTY--- 3'
    3' ---YTTAA   R--- 5'
    AcsI, CfaI,  FsiI, XapI, EcoRI
    AplI 25962 Arthrospira platensis NIES-39 5' CTGCAG
    3' GACGTC
    5' ---CTGCA   G--- 3'
    3' ---G   ACGTC--- 5'
    See this site for a complete list.
    ApyI 93 Arthrobacter pyridinolis 5' CCWGG
    3' GGWCC
    5' ---CC   WGG--- 3'
    3' ---GGW   CC--- 5'
    BciBII, BptI, BseBI, BstNI, BstOI, Bst2UI, Bst38I, Bst100I, MvaI
    ApyPI 14756 Arcanobacterium pyogenes 5' ATCGAC
    3' TAGCTG
    5' ---ATCGACN17NNN   --- 3'
    3' ---TAGCTGN17N   NN--- 5'
    None as of 4 August 2019
    AquI Agmenellum quadruplicatum PR-6 5' CYCGRG
    3' GRGCYC
    5' ---C   YCGRG--- 3'
    3' ---GRGCY   C--- 5'
    BcoI,  BmeT110I,  BsmAI, BsoMAI, BsoBI, BstSI, Eco27kI, OfoI
    AquII 20238 Agmenellum quadruplicatum PR-6 5' GCCGNAC
    3' CGGCNTG
    5' ---GCCGNACN17NNN   --- 3'
    3' ---CGGCNTGN17N   NN--- 5'
    None as of 4 August 2019
    AquIII 20239 Agmenellum quadruplicatum PR-6 5' GAGGAG
    3' CTCCTC
    5' ---GAGGAGN17NNN   --- 3'
    3' ---CTCCTCN17N   NN--- 5'
    BseRI
    AquIV 20240 Agmenellum quadruplicatum PR-6 5' GRGGAAG
    3' CYCCTTC
    5' ---GRGGAAGN16NNN   --- 3'
    3' ---CYCCTTCN16N   NN--- 5'
    None as of 4/9/19
    ArsI 19405 Arthrobacter sp. NTS 5' GACN6TTYG
    3' CTGN6AARC
    5' ---NNNNN   NN7GACN6TTYGN5NNNNNN   --- 3'
    3' ---   NNNNNNN7CTGN6AARCN5N   NNNNN--- 5'
    None as of Oct. 28 2020
    AscI Arthrobacter sp. 5' GGCGCGCC
    3' CCGCGCGG
    5' ---GG   CGCGCC--- 3'
    3' ---CCGCGC   GG--- 5'
     PalAI, SgsI 
    AseI 96 Aquaspirillum serpens 5' ATTAAT
    3' TAATTA
    5' ---AT   TAAT--- 3'
    3' ---TAAT   TA--- 5'
    AsnI, BpoAI, PshBI, Sru4DI, VspI
    AseII 97 Aquaspirillum serpens 5' CCSGG
    3' GGSCC
    5' ---CC   SGG--- 3'
    3' ---GGS   CC--- 5'
    AhaI, AsuC2I, BcnI, CauII, EcoHI, HgiS22I, Kpn49kII, Mgl14481I, NciI
    AsiI Azotobacter sp. 5' GGATCC
    3' CCTAGG
    5' ---G   GATCC--- 3'
    3' ---CCTAG   G--- 5'
    ApaCI, BamHI, BnaI, Bsp4009I, BstI, OkrAI, Pfl8I, SurI, Uba4009I
    AsiAI 2853 Arthrobacter sp. A7359 5' ACCGGT
    3' TGGCCA
    5' ---A   CCGGT--- 3'
    3' ---TGGCC   A--- 5'
    AgeI, AsiGI, BshTI, CsiAI, CspAI, PinAI
    AsiGI 10828 Arthrobacter sp. G 5' ACCGGT
    3' TGGCCA
    5' ---A   CCGGT--- 3'
    3' ---TGGCC   A--- 5'
    AgeI, AsiAI, BshTI, CsiAI, CspAI, PinAI
    AsiSI Arthrobacter sp. 5' GCGATCGC
    3' CGCTAGCG
    5' ---GCGAT   CGC--- 3'
    3' ---CGC   TAGCG--- 5'
     RgaI, SfaAI, , SgfI
    Asi256I 18472 Arthrobacter sp. Ck256 5' GATC
    3' CTAG
    5' ---G   ATC--- 3'
    3' ---CTA   G--- 5'
    See this site for a complete list.
    Asi372I 15803 Arthrobacter sp. Mn372 5' ATGCAT
    3' TACGTA
    5' ---ATGCA   T--- 3'
    3' ---T   ACGTA--- 5'
    Ppu10I, EcoT22I, Mph1103I, NsiI, Zsp2I, BfrBI, Csp68KIII, PinBI, SepI, SspD5II
    AsnI 98 Arthrobacter sp. N-CM 5' ATTAAT
    3' TAATTA
    5' ---AT   TAAT--- 3'
    3' ---TAAT   TA--- 5'
    AseI, BpoAI, PshBI, Sru4DI, VspI
    AspI Achromobacter sp. 699 5' GACNNNGTC
    3' CTGNNNCAG
    5' ---GACN   NNGTC--- 3'
    3' ---CTGNN   NCAG--- 5'
    AtsI, PflFI, PsyI, TelI, Tth111I
    Asp10HI Acidiphilium sp. 10H 5' TTCGAA
    3' AAGCTT
    5' ---TT   CGAA--- 3'
    3' ---AAGC   TT--- 5'
    AsuII, BspT104I, BstBI, CbiI, Csp68KII, PlaII, PpaAI, Ssp1I
    Asp10HII 100 Acidiphilium sp. 10H 5' CCAN5TGG
    3' GGTN5ACC
    5' ---CCANNNN   NTGG--- 3'
    3' ---GGTN   NNNNACC--- 5'
    AccB7I, AcpII, BasI, Esp1396I, PflBI, PflMI, Van91I
    Asp26HI 111 Acidiphilium sp. 26H 5' GAATGC
    3' CTTACG
    5' ---GAATGCN   --- 3'
    3' ---CTTAC   GN--- 5'
    Asp27HI, Asp35HI, Asp36HI, BsaMI, BscCI, BsmI, PctI
    Asp27HI 112 Acidiphilium sp. 27H 5' GAATGC
    3' CTTACG
    5' ---GAATGCN   --- 3'
    3' ---CTTAC   GN--- 5'
    Asp35HI, Asp36HI, Asp40HI,  BmaHI,  BsaMI, BscCI, BsmI
    Asp35HI 117 Acidiphilium sp. 35H 5' GAATGC
    3' CTTACG
    5' ---GAATGCN   --- 3'
    3' ---CTTAC   GN--- 5'
    Asp36HI, Asp40HI, Asp50HI, BsaMI, BscCI, BsmI, Mva1269I
    Asp36HI 118 Acidiphilium sp. 36H 5' GAATGC
    3' CTTACG
    5' ---GAATGCN   --- 3'
    3' ---CTTAC   GN--- 5'
    Asp40HI, Asp50HI,  BmaHI,  BscCI, BsmI, Mva1269I, PctI
    Asp40HI 120 Acidiphilium sp. 40H 5' GAATGC
    3' CTTACG
    5' ---GAATGCN   --- 3'
    3' ---CTTAC   GN--- 5'
    Asp27HI, Asp36HI, Asp50HI, BsaMI, BscCI, BsmI, Mva1269I
    Asp50HI 122 Acidiphilium sp. 50H 5' GAATGC
    3' CTTACG
    5' ---GAATGCN   --- 3'
    3' ---CTTAC   GN--- 5'
    Asp26HI, Asp35HI, Asp36HI, BsaMI, BscCI, BsmI, Mva1269I
    Asp700I Achromobacter sp. 700 5' GAAN4TTC
    3' CTTN4AAG
    5' ---GAANN   NNTTC--- 3'
    3' ---CTTNN   NNAAG--- 5'
    BbvAI, MroXI, PdmI, XmnI
    Asp713I 131 Achromobacter sp. 713 5' CTGCAG
    3' GACGTC
    5' ---CTGCA   G--- 3'
    3' ---G   ACGTC--- 5'
    AjoI, AliAJI, BloHII, CfrA4I, Ecl37kI, MhaAI, PstI, SflI, Srl5DI, YenI
    Asp718I Achromobacter sp. 718 5' GGTACC
    3' CCATGG
    5' ---G   GTACC--- 3'
    3' ---CCATG   G--- 5'
    Acc65I, AhaB8I, KpnI, SthI
    Asp745I Achromobacter sp. 745 5' GGWCC
    3' CCWGG
    5' ---G   GWCC--- 3'
    3' ---CCWG   G--- 5'
    Bme18I, BsrAI, CauI, Eco47I, ErpI, FdiI, SinI, VpaK11AI, VpaK11BI
    AspAI Alcaligenes sp. 5' GGTNACC
    3' CCANTGG
    5' ---G   GTNACC--- 3'
    3' ---CCANTG   G--- 5'
    AcrII, Bse64I, BseT10I, BstEII, BstPI, Eco91I, EcoO65I, PspEI
    AspA2I 6959 Arthrobacter sp. A2 5' CCTAGG
    3' GGATCC
    5' ---C   CTAGG--- 3'
    3' ---GGATC   C--- 5'
    AvrBII, AvrII, BlnI, BspA2I, XmaJI
    AspEI Aureobacterium sp. 5' GACN5GTC
    3' CTGN5CAG
    5' ---GACNNN   NNGTC--- 3'
    3' ---CTGNN   NNNCAG--- 5'
    AhdI,  BmeRI,  BspOVI, DriI, Eam1105I, EclHKI, NruGI
    AspHI Achromobacter sp. H 5' GWGCWC
    3' CWCGWG
    5' ---GWGCW   C--- 3'
    3' ---C   WCGWG--- 5'
    Alw21I, Bbv12I, Bsh45I, BsiHKAI,  Bsm6I,  HgiAI, MspV281I
    AspLEI Arthrobacter sp. LE3860 5' GCGC
    3' CGCG
    5' ---GCG   C--- 3'
    3' ---C   GCG--- 5'
    BspLAI, BstHHI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI, SciNI
    AspMI 2977 Acinetobacter sp. M 5' AGGCCT
    3' TCCGGA
    5' ---AGG   CCT--- 3'
    3' ---TCC   GGA--- 5'
    AatI, Eco147I, GdiI, PceI, SarI, Sru30DI, SseBI, SteI, StuI
    AspMDI Alcaligenes sp. MD1 5' GATC
    3' CTAG
    5' ---   GATC--- 3'
    3' ---CTAG   --- 5'
    BfuCI, Bsp143I, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI
    AspNI Anabaena sp. J3 5' GGNNCC
    3' CCNNGG
    5' ---GGN   NCC--- 3'
    3' ---CCN   NGG--- 5'
     BmiI,  BscBI, BspLI, NlaIV, PspN4I
    AspS9I Arthrobacter sp. S9 5' GGNCC
    3' CCNGG
    5' ---G   GNCC--- 3'
    3' ---CCNG   G--- 5'
    AsuI, AvcI, Bac36I, Bal228I, FmuI, NspIV, PspPI, Sau96I
    AssI 4930 Arthrobacter sp. 5' AGTACT
    3' TCATGA
    5' ---AGT   ACT--- 3'
    3' ---TCA   TGA--- 5'
    Acc113I,  BmcAI, Bpa34I,  DpaI, Eco255I, RflFII, ScaI, ZrmI
    AstWI Anabaena sp. 5' GRCGYC
    3' CYGCRG
    5' ---GR   CGYC--- 3'
    3' ---CYGC   RG--- 5'
    AcyI, AsuIII, BsaHI, BstACI, Hin1I, HgiI, HgiDI, HgiGI, Msp17I
    AsuI Anabaena subcylindrica 5' GGNCC
    3' CCNGG
    5' ---G   GNCC--- 3'
    3' ---CCNG   G--- 5'
    AspS9I, AvcI, CcuI, Cfr13I, FmuI, MaeK81II, Nsp7121I, PspPI, UnbI
    AsuII Anabaena subcylindrica 5' TTCGAA
    3' AAGCTT
    5' ---TT   CGAA--- 3'
    3' ---AAGC   TT--- 5'
    AcpI, Bpu14I, Bsp119I, BspT104I, Csp45I, FspII, LspI, NspV, SfuI
    AsuIII Anabaena subcylindrica 5' GRCGYC
    3' CYGCRG
    5' ---GR   CGYC--- 3'
    3' ---CYGC   RG--- 5'
    AcyI, AsuIII, BbiII, BsaHI, BstACI, Hin1I, HgiHII, Hsp92I, Msp17I
    AsuC2I 2901 Actinobacillus suis C2 5' CCSGG
    3' GGSCC
    5' ---CC   SGG--- 3'
    3' ---GGS   CC--- 5'
    AhaI, AseII, BcnI, Eco1831I, EcoHI, Kpn49kII, Mgl14481I, NciI
    AsuHPI 2811 Actinobacillus suis HP 5' GGTGA
    3' CCACT
    5' ---GGTGAN6NN   --- 3'
    3' ---CCACTN6N   N--- 5'
    HphI, SspD5I
    AsuNHI Actinobacillus suis NH 5' GCTAGC
    3' CGATCG
    5' ---G   CTAGC--- 3'
    3' ---CGATC   G--- 5'
    AceII, BmtI,  BspOI,  LlaG2I, NheI, PstNHI
    AtsI Aureobacterium testaceum 4842 5' GACNNNGTC
    3' CTGNNNCAG
    5' ---GACN   NNGTC--- 3'
    3' ---CTGNN   NCAG--- 5'
    AspI, PflFI, PsyI, TelI, Tth111I
    AvaI Anabaena variabilis 5' CYCGRG
    3' GRGCYC
    5' ---C   YCGRG--- 3'
    3' ---GRGCY   C--- 5'
    AquI, Ama87I, BsiHKCI, BsoBI, BspLU4I, Eco88I, NspIII, PlaAI
    AvaII Anabaena variabilis 5' GGWCC
    3' CCWGG
    5' ---G   GWCC--- 3'
    3' ---CCWG   G--- 5'
    Bme18I, Bme216I, BsrAI, Eco47I, Kzo49I, Psp03I, SmuEI, VpaK11AI
    AvcI Actinomyces violaceoniger cristalomycini 5' GGNCC
    3' CCNGG
    5' ---G   GNCC--- 3'
    3' ---CCNG   G--- 5'
    AspS9I, BshKI, BsiZI, Bsp1894I, BspBII, BspF4I, Bsu54I, Pde12I
    AviII Anabaena variabillis (halle) 5' TGCGCA
    3' ACGCGT
    5' ---TGC   GCA--- 3'
    3' ---ACG   CGT--- 5'
    Acc16I, AosI, FdiII, FspI, MstI, NsbI, PamI, Pun14627I
    AvrII 173 Anabaena variabilis UW 5' CCTAGG
    3' GGATCC
    5' ---C   CTAGG--- 3'
    3' ---GGATC   C--- 5'
    AspA2I, AvrBII, BlnI, BspA2I, XmaJI
    AvrBII 171 Arthrobacter variabilis 5' CCTAGG
    3' GGATCC
    5' ---C   CTAGG--- 3'
    3' ---GGATC   C--- 5'
    AspA2I, AvrII, BlnI, BspA2I, XmaJI
    AxyI 174 Acetobacter xylinus 5' CCTNAGG
    3' GGANTCC
    5' ---CC   TNAGG--- 3'
    3' ---GGANT   CC--- 5'
    AocI, BliHKI, Bse21I, BspR7I, Bsu36I, CvnI, Eco81I, Lmu60I

    § An HF version of this enzyme is available


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