Enzyme
|
PDB code
|
Source
|
Recognition sequence
|
Cut
|
Isoschizomers
|
BsaI
|
|
Bacillus stearothermophilus 20241
|
5' GGTCTC
3' CCAGAG
|
5' ---GGTCTCN NNNN--- 3'
3' ---CCAGAGNNNNN --- 5'
|
Bso31I, BspTNI, Eco31I
|
Bsa29I
|
|
Bacillus sp. 29
|
5' ATCGAT
3' TAGCTA
|
5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5'
|
AagI, BanIII, BavCI, BseCI, BspDI, Bsu15I, BsuTUI, ClaI, BbvAII
|
BsaAI
|
|
Bacillus stearothermophilus G668
|
5' YACGTR
3' RTGCAY
|
5' ---YAC GTR--- 3'
3' ---RTG CAY--- 5'
|
|
BsaBI
|
|
Bacillus stearothermophilus B674
|
5' GATN4ATC
3' CTAN4TAG
|
5' ---GATNN NNATC--- 3'
3' ---CTANN NNTAG--- 5'
|
|
BsaHI
|
|
Bacillus stearothermophilus CPW11
|
5' GRCGYC
3' CYGCRG
|
5' ---GR CGYC--- 3'
3' ---CYGC RG--- 5'
|
AcyI, AhaII, AosII, AstWI, AsuIII, BbiII, BstACI, HgiI, PamII
|
BsaJI
|
|
Bacillus stearothermophilus J695
|
5' CCNNGG
3' GGNNCC
|
5' ---C CNNGG--- 3'
3' ---GGNNC C--- 5'
|
|
BsaMI
|
|
Bacillus stearothermophilus M293
|
5' GAATGC
3' CTTACG
|
5' ---GAATGCN --- 3'
3' ---CTTAC GN--- 5'
|
Asp26HI, Asp35HI, Asp36HI, Asp50HI, BsaMI, BsmI, Mva1269I
|
BsaOI
|
|
Bacillus stearothermophilus O-122
|
5' CGRYCG
3' GCYRGC
|
5' ---CGRY CG--- 3'
3' ---GC YRGC--- 5'
|
|
BsaWI
|
|
Bacillus stearothermophilus W1718
|
5' WCCGGW
3' WGGCCW
|
5' ---W CCGGW--- 3'
3' ---WGGCC W--- 5'
|
|
BsaXI
|
|
Bacillus stearothermophilus 25B
|
5' ACN5CTCC
3' TGN5GAGG
|
5' ---ACN5CTCCN6NNNN --- 3'
3' ---TGN5GAGGN6N NNN--- 5'
|
|
BscI
|
|
Bacillus sp.
|
5' ATCGAT
3' TAGCTA
|
5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5'
|
BanIII, BavCI, Bci29I, BdiI, Bli41I, BliAI, LplI, PgaI, SpmI
|
Bsc4I
|
|
Bacillus schlegelii 4
|
5' CCN7GG
3' GGN7CC
|
5' ---CCNNNNN NNGG--- 3'
3' ---GGNN NNNNNCC--- 5'
|
|
Bsc91I
|
|
Bacillus sp.
|
5' GAAGAC
3' CTTCTG
|
5' ---GAAGACNN NNNN--- 3'
3' ---CTTCTGNNNNNN --- 5'
|
|
Bsc107I
|
|
Bacillus schlegelii 107
|
5' CCN7GG
3' GGN7CC
|
5' ---CCNNNNN NNGG--- 3'
3' ---GGNN NNNNNCC--- 5'
|
|
BscAI
|
|
Bacillus schlegelii S3
|
5' GCATC
3' CGTAG
|
5' ---GCATCNNNN NN--- 3'
3' ---CGTAGNNNNNN --- 5'
|
|
BscBI
|
|
Bacillus sp. A11
|
5' GGNNCC
3' CCNNGG
|
5' ---GGN NCC--- 3'
3' ---CCN NGG--- 5'
|
AspNI,
|
BmiI,
|
BspLI, NlaIV,
|
PspN4I
|
|
BscCI
|
|
Bacillus sp. 2G
|
5' GAATGC
3' CTTACG
|
5' ---GAATGCN --- 3'
3' ---CTTAC GN--- 5'
|
Asp26HI, Asp27HI, Asp35HI, Asp36HI, BsaMI, BscCI, PctI
|
BscFI
|
|
Bacillus sp. JY391
|
5' GATC
3' CTAG
|
5' --- GATC--- 3'
3' ---CTAG --- 5'
|
Bfi57I, Bsp143I, BspJI, BtkII, CviAI, Kzo9I, NlaII, SsiBI
|
Bse1I
|
|
Bacillus stearothermophilus 1
|
5' ACTGG
3' TGACC
|
5' ---ACTGGN --- 3'
3' ---TGAC CN--- 5'
|
|
Bse8I
|
|
Bacillus sp. 8
|
5' GATN4ATC
3' CTAN4TAG
|
5' ---GATNN NNATC--- 3'
3' ---CTANN NNTAG--- 5'
|
|
Bse15I
|
|
Bacillus sp. 15
|
5' CYCGRG
3' GRGCYC
|
5' ---C YCGRG--- 3'
3' ---GRGCY C--- 5'
|
Ama87I, AvaI, BcoI, BsoBI, BstSI, Eco88I, NspSAI, OfoI
|
Bse16I
|
|
Bacillus sp. 16
|
5' CCWGG
3' GGWCC
|
5' ---CC WGG--- 3'
3' ---GGW CC--- 5'
|
AeuI, BseBI, BsiLI, BstNI, BstM6I, BthDI, BthEI, EcoRII, MvaI
|
Bse17I
|
|
Bacillus sp. 17
|
5' CCWGG
3' GGWCC
|
5' ---CC WGG--- 3'
3' ---GGW CC--- 5'
|
AeuI, BseBI, Bse16I, Bst2I, BstNI, Bst38I, Bst100I, Psp6I, PspGI
|
Bse21I
|
|
Bacillus sp. 21
|
5' CCTNAGG
3' GGANTCC
|
5' ---CC TNAGG--- 3'
3' ---GGANT CC--- 5'
|
AxyI, BliHKI, BspR7I, Bsu36I, Eco81I, MstII, OxaNI, SshAI
|
Bse24I
|
|
Bacillus sp. 24
|
5' CCWGG
3' GGWCC
|
5' ---CC WGG--- 3'
3' ---GGW CC--- 5'
|
AeuI, BseBI, Bse16I, Bst2I, BstNI, Bst38I, Bst100I, Psp6I, PspGI
|
Bse64I
|
|
Bacillus sp. 64
|
5' GGTNACC
3' CCANTGG
|
5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5'
|
AspAI, Bse64I, BseT9I, BstPI, EcaI, Eci125I, EcoO65I, NspSAII
|
Bse118I
|
|
Bacillus sp. 118
|
5' RCCGGY
3' YGGCCR
|
5' ---R CCGGY--- 3'
3' ---YGGCC R--- 5'
|
|
Bse634I
|
1KNV
|
Bacillus sp. 634
|
5' RCCGGY
3' YGGCCR
|
5' ---R CCGGY--- 3'
3' ---YGGCC R--- 5'
|
|
BseAI
|
|
Bacillus sphaericus
|
5' TCCGGA
3' AGGCCT
|
5' ---T CCGGA--- 3'
3' ---AGGCC T--- 5'
|
AccIII, Aor13HI, BlfI, BsiMI, Bsp13I, BspMII, CauB3I, Kpn2I
|
BseBI
|
|
Bacillus stearothermophilus
|
5' CCWGG
3' GGWCC
|
5' ---CC WGG--- 3'
3' ---GGW CC--- 5'
|
AjnI, AorI, Bse17I, Bst1I, BstOI, Bst2UI, EcoRII, MvaI, SleI, ZanI
|
BseCI
|
|
Bacillus sp.
|
5' ATCGAT
3' TAGCTA
|
5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5'
|
AagI, BanIII, BavCI, Bsa29I, BspDI, Bsu15I, BsuTUI, ClaI, LcaI
|
BseDI
|
|
Bacillus stearothermophilus RFL1434
|
5' CCNNGG
3' GGNNCC
|
5' ---C CNNGG--- 3'
3' ---GGNNC C--- 5'
|
|
Bse3DI
|
|
Bacillus stearothermophilus 3D
|
5' GCAATG
3' CGTTAC
|
5' ---GCAATGNN --- 3'
3' ---CGTTAC NN--- 5'
|
|
BseGI
|
|
Bacillus stearothermophilus Vs 34-031
|
5' GGATG
3' CCTAC
|
5' ---GGATGNN --- 3'
3' ---CCTAC NN--- 5'
|
|
BseJI
|
|
Bacillus stearothermophilus Tsp5
|
5' GATN4ATC
3' CTAN4TAG
|
5' ---GATNN NNATC--- 3'
3' ---CTANN NNTAG--- 5'
|
|
BseKI
|
|
Bacillus stearothermophilus Ra3-212
|
5' GCAGC
3' CGTCG
|
5' ---GCAGCN7N NNNN--- 3'
3' ---CGTCGN7NNNNN --- 5'
|
AlwXI, BbvI, BseXI, Bsp423I, Bst12I, Bst71I, BstV1I
|
BseLI
|
|
Bacillus stearothermophilus LK3-551
|
5' CCN7GG
3' GGN7CC
|
5' ---CCNNNNN NNGG--- 3'
3' ---GGNN NNNNNCC--- 5'
|
|
BseMI
|
|
Bacillus stearothermophilus Isl 15-111
|
5' GCAATG
3' CGTTAC
|
5' ---GCAATGNN --- 3'
3' ---CGTTAC NN--- 5'
|
|
BseMII
|
|
Bacillus stearothermophilus Isl 15-111
|
5' CTCAG
3' GAGTC
|
5' ---CTCAGN7NNN --- 3'
3' ---GAGTCN7N NN--- 5'
|
|
BseNI
|
|
Bacillus sp. N
|
5' ACTGG
3' TGACC
|
5' ---ACTGGN --- 3'
3' ---TGAC CN--- 5'
|
|
BsePI
|
|
Bacillus stearothermophilus P6
|
5' GCGCGC
3' CGCGCG
|
5' ---G CGCGC--- 3'
3' ---CGCGC G--- 5'
|
|
BseQI
|
|
Bacillus sp. Q
|
5' GGCC
3' CCGG
|
5' ---GG CC--- 3'
3' ---CC GG--- 5'
|
|
BseRI
|
|
Bacillus sp. R
|
5' GAGGAG
3' CTCCTC
|
5' ---GAGGAGN7NNN --- 3'
3' ---CTCCTCN7N NN--- 5'
|
|
BseSI
|
|
Bacillus stearothermophilus Jo-553
|
5' GKGCMC
3' CMCGKG
|
5' ---GKGCM C--- 3'
3' ---C MCGKG--- 5'
|
|
BseT9I
|
|
Bacillus sp. T9
|
5' GGTNACC
3' CCANTGG
|
5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5'
|
AcrII, Bse64I, BstEII, BstT9I, EcaI, Eci125I, EcoO65I, NspSAII, PspEI
|
BseT10I
|
|
Bacillus sp. T10
|
5' GGTNACC
3' CCANTGG
|
5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5'
|
AcrII, AspAI, BstEII, BstT10I, BstPI, EcoO128I, EcoO65I, PspEI
|
BseXI
|
|
Bacillus stearothermophilus Ra3-212
|
5' GCAGC
3' CGTCG
|
5' ---GCAGCN7N NNNN--- 3'
3' ---CGTCGN7NNNNN --- 5'
|
AlwXI, BbvI, BseKI, Bsp423I, Bst12I, Bst71I, BstV1I
|
BseX3I
|
|
Bacillus stearothermophilus X3
|
5' CGGCCG
3' GCCGGC
|
5' ---C GGCCG--- 3'
3' ---GCCGG C--- 5'
|
AaaI, BstZI, EagI, EclXI, Eco52I, SenPT16I, XmaIII
|
BseYI
|
|
Bacillus sp. 2521
|
5' CCCAGC
3' GGGTCG
|
5' ---C CCAGC--- 3'
3' ---GGGTC G--- 5'
|
|
BseZI
|
|
Bacillus sp. Z
|
5' CTCTTC
3' GAGAAG
|
5' ---CTCTTCN NNN--- 3'
3' ---GAGAAGNNNN --- 5'
|
|
BsgI
|
|
Bacillus sphaericus B922
|
5' GTGCAG
3' CACGTC
|
5' ---GTGCAGN12NNNN --- 3'
3' ---CACGTCN12NN NN--- 5'
|
|
BshI
|
|
Bacillus sphaericus
|
5' GGCC
3' CCGG
|
5' ---GG CC--- 3'
3' ---CC GG--- 5'
|
|
Bsh45I
|
|
Bacillus sphaericus 45
|
5' GWGCWC
3' CWCGWG
|
5' ---GWGCW C--- 3'
3' ---C WCGWG--- 5'
|
Alw21I, AspHI, Bbv12I, BsiHKAI,
|
HgiAI,
|
HpyF46II,
|
MspV281I
|
|
Bsh1236I
|
|
Bacillus sphaericus RFL1236
|
5' CGCG
3' GCGC
|
5' ---CG CG--- 3'
3' ---GC GC--- 5'
|
AccII, BceBI, BspFNI, Bsp50I, Csp68KVI, FauBII, MvnI, SelI
|
Bsh1285I
|
|
Bacillus sphaericus RFL1285
|
5' CGRYCG
3' GCYRGC
|
5' ---CGRY CG--- 3'
3' ---GC YRGC--- 5'
|
|
Bsh1365I
|
|
Bacillus sphaericus RFL1365
|
5' GATN4ATC
3' CTAN4TAG
|
5' ---GATNN NNATC--- 3'
3' ---CTANN NNTAG--- 5'
|
|
BshFI
|
|
Bacillus sphaericus
|
5' GGCC
3' CCGG
|
5' ---GG CC--- 3'
3' ---CC GG--- 5'
|
|
BshGI
|
|
Bacillus sphaericus
|
5' CCWGG
3' GGWCC
|
5' ---CC WGG--- 3'
3' ---GGW CC--- 5'
|
AeuI, BseBI, Bse16I, Bst2I, BstNI, Bst38I, Bst100I, PspGI, SspAI
|
BshKI
|
|
Bacillus sphaericus
|
5' GGNCC
3' CCNGG
|
5' ---G GNCC--- 3'
3' ---CCNG G--- 5'
|
AspS9I, Bac36I, BavAII, Bsp1894I, BspBII, CcuI, MaeK81II, Pde12I
|
BshNI
|
|
Bacillus sphaericus TK-45
|
5' GGYRCC
3' CCRYGG
|
5' ---G GYRCC--- 3'
3' ---CCRYG G--- 5'
|
BanI, BbvBI, BspT107I, Eco64I, HgiCI, HgiHI, MspB4I, PfaAI
|
BshTI
|
|
Bacillus sphaericus Jo22-024
|
5' ACCGGT
3' TGGCCA
|
5' ---A CCGGT--- 3'
3' ---TGGCC A--- 5'
|
AgeI, AsiAI,
|
AsiGI,
|
CsiAI
|
CspAI, PinAI
|
|
BsiI
|
|
Bacillus sphaericus
|
5' CACGAG
3' GTGCTC
|
5' ---C ACGAG--- 3'
3' ---GTGCT C--- 5'
|
|
BsiBI
|
|
Bacillus sp.
|
5' GATN4ATC
3' CTAN4TAG
|
5' ---GATNN NNATC--- 3'
3' ---CTANN NNTAG--- 5'
|
|
BsiCI
|
|
Bacillus sp.
|
5' TTCGAA
3' AAGCTT
|
5' ---TT CGAA--- 3'
3' ---AAGC TT--- 5'
|
AcpI, AsuII, Bpu14I, BspT104I, Csp45I, FspII, NspV, PpaAI, SfuI
|
BsiEI
|
|
Bacillus sp.
|
5' CGRYCG
3' GCYRGC
|
5' ---CGRY CG--- 3'
3' ---GC YRGC--- 5'
|
|
BsiHKAI
|
|
Bacillus stearothermophilus
|
5' GWGCWC
3' CWCGWG
|
5' ---GWGCW C--- 3'
3' ---C WCGWG--- 5'
|
Alw21I, AspHI, Bbv12I, Bsh45I,
|
|
BsiHKCI
|
|
Bacillus sp. HKC
|
5' CYCGRG
3' GRGCYC
|
5' ---C YCGRG--- 3'
3' ---GRGCY C--- 5'
|
AquI, BcoI, Bse15I, BspLU4I, Eco88I, Nli3877I, PlaAI, PunAI
|
BsiKI
|
|
Bacillus sp.
|
5' GGTNACC
3' CCANTGG
|
5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5'
|
|
BsiLI
|
|
Bacillus sp.
|
5' CCWGG
3' GGWCC
|
5' ---CC WGG--- 3'
3' ---GGW CC--- 5'
|
AglI, BseBI, Bse17I, Bst2I, BstNI, Bst38I, Bst100I, EcoRII, MvaI
|
BsiMI
|
|
Bacillus sp.
|
5' TCCGGA
3' AGGCCT
|
5' ---T CCGGA--- 3'
3' ---AGGCC T--- 5'
|
AccIII, BbvAIII, BlfI, Bsp13I, BspMII, Bsu23I, Kpn2I, PinBII
|
BsiQI
|
|
Bacillus sp.
|
5' TGATCA
3' ACTAGT
|
5' ---T GATCA--- 3'
3' ---ACTAG T--- 5'
|
AbaI, BclI, BspXII,
|
BstT7I, FbaI, Ksp22I,
|
ParI
|
|
BsiSI
|
|
Bacillus sp.
|
5' CCGG
3' GGCC
|
5' ---C CGG--- 3'
3' ---GGC C--- 5'
|
|
BsiWI
|
|
Bacillus sp.
|
5' CGTACG
3' GCATGC
|
5' ---C GTACG--- 3'
3' ---GCATG C--- 5'
|
|
BsiXI
|
|
Bacillus sp. X
|
5' ATCGAT
3' TAGCTA
|
5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5'
|
BscI, BsiXI, Bsp106I, BspDI, Bsu15I, BsuTUI, ClaI, Rme21I
|
BsiYI
|
|
Bacillus sp.
|
5' CCN7GG
3' GGN7CC
|
5' ---CCNNNNN NNGG--- 3'
3' ---GGNN NNNNNCC--- 5'
|
|
BsiZI
|
|
Bacillus sp.
|
5' GGNCC
3' CCNGG
|
5' ---G GNCC--- 3'
3' ---CCNG G--- 5'
|
AspS9I, Bac36I, BavAII, BshKI, BspBII, CcuI, MaeK81II, Pde12I
|
BslI
|
|
Bacillus sp.
|
5' CCN7GG
3' GGN7CC
|
5' ---CCNNNNN NNGG--- 3'
3' ---GGNN NNNNNCC--- 5'
|
|
BslFI
|
|
Bacillus stearothermophilus FI
|
5' GGGAC
3' CCCTG
|
5' ---GGGACN8NN NNNN--- 3'
3' ---CCCTGN8NNNNNN --- 5'
|
|
BsmI
|
|
Bacillus stearothermophilus NUB
|
5' GAATGC
3' CTTACG
|
5' ---GAATGCN --- 3'
3' ---CTTAC GN--- 5'
|
Asp26HI, Asp36HI, Asp40HI, BmaHI, BscCI, Mva1269I, PctI
|
BsmAI
|
|
Bacillus stearothermophilus A664
|
5' GTCTC
3' CAGAG
|
5' ---GTCTCN NNNN--- 3'
3' ---CAGAGNNNNN --- 5'
|
Alw26I,
|
BcoDI, BscQII,
|
BsoMAI,
|
BstMAI
|
|
BsmBI
|
|
Bacillus stearothermophilus B61
|
5' CGTCTC
3' GCAGAG
|
5' ---CGTCTCN NNNN--- 3'
3' ---GCAGAGNNNNN --- 5'
|
|
BsmFI
|
|
Bacillus stearothermophilus F
|
5' GGGAC
3' CCCTG
|
5' ---GGGACN8NN NNNN--- 3'
3' ---CCCTGN8NNNNNN --- 5'
|
|
BsmSI
|
|
Bacillus stearothermophilus CP114
|
5' CCWWGG
3' GGWWCC
|
5' ---C CWWGG--- 3'
3' ---GGWWC C--- 5'
|
|
Bso31I
|
|
Bacillus stearothermophilus E31
|
5' GGTCTC
3' CCAGAG
|
5' ---GGTCTCN NNNN--- 3'
3' ---CCAGAGNNNNN --- 5'
|
|
BsoBI
|
1DC1
|
Bacillus stearothermophilus JN2091
|
5' CYCGRG
3' GRGCYC
|
5' ---C YCGRG--- 3'
3' ---GRGCY C--- 5'
|
AquI, BcoI, BsiHKCI, BspLU4I, Eco88I, Nli3877I, PlaAI, PunAI
|
BsoCI
|
|
Bacillus stearothermophilus AU891
|
5' GDGCHC
3' CHCGDG
|
5' ---GDGCH C--- 3'
3' ---C HCGDG--- 5'
|
AocII, BmyI, Bsp1286I, BspLS2I, MhlI, NspII, SduI
|
BsoFI
|
|
Bacillus stearothermophilus F66
|
5' GCNGC
3' CGNCG
|
5' ---GC NGC--- 3'
3' ---CGN CG--- 5'
|
|
BsoMAI
|
|
Bacillus stearothermophilus MA
|
5' GTCTC
3' CAGAG
|
5' ---GTCTCN NNNN--- 3'
3' ---CAGAGNNNNN --- 5'
|
Alw26I,
|
BcoDI, BscQII,
|
BsmAI,
|
BstMAI
|
|