Protein OS-9 is a protein that in humans is encoded by the OS9 gene.
Function
This gene encodes a protein that is highly expressed in osteosarcomas. This protein binds to the hypoxia-inducible factor 1 (HIF-1), a key regulator of the hypoxic response and angiogenesis, and promotes the degradation of one of its subunits. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Model organisms
Model organisms have been used in the study of OS9 function. A conditional knockout mouse line called Os9tm1a(EUCOMM)Wtsi was generated at the Wellcome Trust Sanger Institute. Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. Additional screens performed: - In-depth immunological phenotyping
Os9 knockout mouse phenotype
| Characteristic |
Phenotype
|
| All data available at.{
|
| Insulin |
Normal
|
| Homozygous viability at P14 |
Normal
|
| Homozygous Fertility |
Normal
|
| General Observations |
Abnormal
|
| Body weight |
Normal
|
| Neurological assessment |
Normal
|
| Grip strength |
Normal
|
| Dysmorphology |
Normal
|
| Indirect calorimetry |
Normal
|
| Glucose tolerance test |
Normal
|
| Auditory brainstem response |
Normal
|
| DEXA |
Normal
|
| Radiography |
Normal
|
| Eye morphology |
Normal
|
| Clinical chemistry |
Normal
|
|
Haematology 16 Weeks |
Normal
|
| Peripheral blood leukocytes 16 Weeks |
Normal
|
| Heart weight |
Normal
|
|
Salmonella infection |
Normal
|
| Spleen Immunophenotyping |
Normal
|
| Mesenteric Lymph Node Immunophenotyping |
Normal
|
| Anti-nuclear Antibody Assay |
Normal
|
| Epidermal Immune Composition |
Normal
|
| Influenza Challenge |
Normal
|
Further reading
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Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T, Yamashita R, Yamamoto J, Sekine M, Tsuritani K, Wakaguri H, Ishii S, Sugiyama T, Saito K, Isono Y, Irie R, Kushida N, Yoneyama T, Otsuka R, Kanda K, Yokoi T, Kondo H, Wagatsuma M, Murakawa K, Ishida S, Ishibashi T, Takahashi-Fujii A, Tanase T, Nagai K, Kikuchi H, Nakai K, Isogai T, Sugano S (Jan 2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Research. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
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Baek JH, Mahon PC, Oh J, Kelly B, Krishnamachary B, Pearson M, Chan DA, Giaccia AJ, Semenza GL (Feb 2005). "OS-9 interacts with hypoxia-inducible factor 1alpha and prolyl hydroxylases to promote oxygen-dependent degradation of HIF-1alpha". Molecular Cell. 17 (4): 503–12. doi:10.1016/j.molcel.2005.01.011. PMID 15721254.
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Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM (Jul 2004). "Functional proteomics mapping of a human signaling pathway". Genome Research. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748.
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Vigneron N, Ooms A, Morel S, Degiovanni G, Van Den Eynde BJ (Jul 2002). "Identification of a new peptide recognized by autologous cytolytic T lymphocytes on a human melanoma". Cancer Immunity. 2: 9. PMID 12747754.
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Litovchick L, Friedmann E, Shaltiel S (Sep 2002). "A selective interaction between OS-9 and the carboxyl-terminal tail of meprin beta". The Journal of Biological Chemistry. 277 (37): 34413–23. doi:10.1074/jbc.M203986200. PMID 12093806.
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Friedmann E, Salzberg Y, Weinberger A, Shaltiel S, Gerst JE (Sep 2002). "YOS9, the putative yeast homolog of a gene amplified in osteosarcomas, is involved in the endoplasmic reticulum (ER)-Golgi transport of GPI-anchored proteins". The Journal of Biological Chemistry. 277 (38): 35274–81. doi:10.1074/jbc.M201044200. PMID 12077121.
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Nakayama T, Yaoi T, Kuwajima G, Yoshie O, Sakata T (Jun 1999). "Ca2(+)-dependent interaction of N-copine, a member of the two C2 domain protein family, with OS-9, the product of a gene frequently amplified in osteosarcoma". FEBS Letters. 453 (1–2): 77–80. doi:10.1016/S0014-5793(99)00700-0. PMID 10403379. S2CID 1290433.
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Kimura Y, Nakazawa M, Yamada M (May 1998). "Cloning and characterization of three isoforms of OS-9 cDNA and expression of the OS-9 gene in various human tumor cell lines". Journal of Biochemistry. 123 (5): 876–82. doi:10.1093/oxfordjournals.jbchem.a022019. PMID 9562620.
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Elkahloun AG, Krizman DB, Wang Z, Hofmann TA, Roe B, Meltzer PS (Jun 1997). "Transcript mapping in a 46-kb sequenced region at the core of 12q13.3 amplification in human cancers". Genomics. 42 (2): 295–301. doi:10.1006/geno.1997.4727. PMID 9192850.
