Spot-tag
Подписчиков: 0, рейтинг: 0
A Spot-tag is a 12-amino acid peptide tag recognized by a single-domain antibody (sdAb, or nanobody). Due to the small size of a Spot-tag (12 amino acids) and the robust Spot-nanobody (14.7 kD) that specifically binds to Spot-tagged proteins, Spot-tag can be used for multiple capture and detection applications: Immunoprecipitation, affinity purification, immunofluorescence, and super-resolution microscopy.Recombinant proteins can be engineered to express the Spot-tag.
Spot-tag Sequence
Amino acid sequence
PDRVRAVSHWSS
Codon optimized DNA sequence
| Human | CCA GAC CGC GTG CGC GCC GTG AGC CAT TGG AGC AGC |
| S. cerevisiae | CCA GAT AGA GTT AGA GCT GTT TCT CAT TGG TCT TCT |
| E. coli | CCG GAT CGC GTG CGC GCA GTC TCT CAC TGG AGC AGC |
See also
| length<10aa | |
|---|---|
| 10aa<length<50aa | |
| length>50aa | |